Mechanism of Extracellular Vesicles Derived fromMSCs in Implantation: A Meta-Transcriptomic Analysis

Background: A growing body of evidence has shown that extracellularvesicles (EV) can be efficient on ۳ s teps of embryoimplantation however there is a significant unmet need to understand comprehensively how EVs derived from mesenchymals tem cell (MSC) are involved in the embryo implantation.The objective of the research is to gain a better insight into EVstherapeutic mechanisms.Materials and Methods: MicroRNA profiles of extracellular vesicles from MSC were achieved by searching in literature andGEO databases. The s tudies which applied well-characterizedMSCs and their EVs based on ISCT and ISEV guidelines, respectively,were selected for further analysis.To provide a functional biological network with target genesof common miRNAs, the S tRING software was applied andthen visualized using Cytoscape ۳.۷.۱. To analyze the networkand find significant biological clus ters, the Molecular ComplexDetection (MCODE) Cytoscape plugin was used. Functionalenrichment and gene ontology (GO) analysis were performedusing DAVID Bioinformatic Resources.Results: According to the Venn diagram, only a small portionof miRNAs are overlapped among protein lis ts. These sharedcommon miRNAs were associated with signaling pathwayssuch as “IL-۴, IL-۱۳ signaling pathway”, “NOTCH signaling”,“PIP۳ activates AKT signaling” as well as several other pathwaysrelated to reproduction, supporting that EVs from MSCwas expected to positively impact on embryo development.Conclusion: The central themes of biological process of hubgenes such as the “in utero embryonic development”, “embryoimplantation”, and “pros tate gland developement” confirmedthe conclusion that MSC derived EVs play a key role in implantationand treatment of reproductive diseases.

کلیدواژه ها:

Bioinformatics Analysis ، Extracellular Vesicles ، Implantation ، Mesenchymal S tem Cells ، MicroRNA ، ReproductiveDisease ، Transcriptomics