Association between The C۱۶۱T Polymorphism ofThe PPARγ Gene and As thenozoospermia Infertile MenReferred to Royan Ins titute

Background: Peroxisome proliferator activator receptor gamma(PPARγ) is a nuclear transcription factor, which mainlyregulate the expression of target genes involved in lipid andenergy metabolism. Energy from glucose and fat metabolismmediated by PPARγ signaling is required for sperm motility, affectingmale fertility. In the tes tis, PPARγ protein is detected atSertoli cells & spermatocytes. One of the mos t important singlenucleotide polymorphisms of this gene is C۱۶۱T, which is associatedwith decreased transcription of PPARγ. This polymorphismis in linkage disequilibrium with other mutations of thegene that regulate the activity of PPARγ and its association withas thenospermia has not been s tudied so far.Materials and Methods: In this case-control s tudy, genomicDNA was extracted from blood samples of ۶۰ infertile menwith as thenospermia and ۷۰ fertile controls. PCR-RFLP (Restriction Fragment Length Polymorphism) with Pml۱ enzyme was performed to screen the aforementioned polymorphism andthe results were confirmed by Sanger sequencing.Results: Results showed that among ۶۰ infertile men, ۷۳.۳۳%had CC genotype, ۲۵.۰۰% had CT genotype and ۱.۶۶% hadTT genotype. Of the ۷۰ fertile controls, ۷۲.۸۵% had CC genotype,۲۷.۱۴% had CT genotype and ۰% had TT genotype. Theobtained data showed no significant association between twogroups (P=۰.۷۴۸).Conclusion: It is concluded that C۱۶۱T polymorphism cannotbe a risk factor for as thenozoospermia infertile men in the currentsample size of Iranian populations.

کلیدواژه ها:

As thenozoospermia ، C۱۶۱T ، Polymorphism ، PPARγ