Metformin Reduces Cyclophosphamide-InducedOvarian Damage by Maintaining Ovarian Reserve

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 39

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شناسه ملی سند علمی:

RROYAN23_145

تاریخ نمایه سازی: 17 دی 1401

چکیده مقاله:

Background: Activation of primordial follicles appears to be acommon side effect of cyclophosphamide for patients receivingchemotherapy through over-activation of the AKT/mTOR pathway.This has a detrimental effect on fertility since the densityand proportion of intact primordial follicles are important factorsin the reproductive health of women. In this s tudy, we usedmetformin, a drug used to treat type ۲ diabetes as well as polycystic ovary syndrome, as an AKT/mTOR pathway inhibitor.Materials and Methods: Prepubertal NMRI female mice weredivided into three groups: Control, cyclophosphamide (cyc),and cyclophosphamide treated with metformin (cyc-met). Inthe cyc group, in every three days, one dose of ۶۵mg/kg cyclophosphamidewas injected intraperitoneally for three times.In cyc-met group, two days before s tarting cyclophosphamideinjection, metformin was injected in a dose of ۱۵۰ mg/kg for۱۱ consecutive days. The mice were sacrificed ۲۴ hours afterthe las t metformin injection, and the ovaries were gathered forhis tological evaluation.Results: Degeneration of primordial and growing follicles wassimilar between all groups. The counting of the primordial folliclenumber showed that the ovarian reserve in the cyc-metgroup was similar to that of the control group, and it had asignificant increase compared to the cyclophosphamide group(P=۰.۰۰۰۵). On the other hand, the number of growing folliclesin the cyc-met group significantly reduced compared to the cycgroup (P=۰.۰۰۰۲).Conclusion: The presented data show that metformin may preventcyclophosphamide-induced ovarian damage, which overactivesthe AKT/mTOR pathway

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نویسندگان

N Zatalian

Faculty of Basic Sciences and Advanced Technologies in Biology,Department of Molecular Cell Biology-Genetics, University ofScience and Culture, Tehran, Iran

A Dalman

Department of Embryology, Reproductive Biomedicine ResearchCenter, Royan Ins titute for Reproductive Biomedicine, ACECR,Tehran, Iran

P Afsharian

Department of Genetics, Reproductive Biomedicine ResearchCenter, Royan Ins titute for Reproductive Biomedicine, ACECR,Tehran, Iran

H Gourabi

Department of Genetics, Reproductive Biomedicine ResearchCenter, Royan Ins titute for Reproductive Biomedicine, ACECR,Tehran, Iran