Increased Expression of Klotho Gene in CumulusCells of Women with Polycys tic Ovary Syndrome

Background: Polycys tic ovary syndrome (PCOS) is the mainreason for anovulatory infertility and the mos t common endocrinopathyaffecting women of reproductive age. Due to the complicated,multifactorial, and polygenic nature of PCOS, variationin phenotypic expression among individuals has been recognized.However, little is elucidated about its genetic and molecular basis.Klotho (Kl) is an antiaging gene concerned with mammals'aging process that accomplishes various functions from an obligatoryco-receptor for the fibroblas t growth factor family to ionchannels/transporters modulation. Klotho plays a pivotal role inearthshaking signaling pathways like PI۳K/Akt/mTOR, insulin/IGF-۱, Wnt۱, and MAP Kinase. In a recent s tudy, Knockdownof the Klotho gene normalized signaling mentioned pathways ingranulosa cells (GCs) of patients with PCOS and the ovarian tissuesof PCOS rats. Moreover, the role of klotho protein in theapoptosis of GCs in patients with PCOS was identified.Materials and Methods: Two groups of women (۲۰ to ۳۵ yearsold) referred to the Royan Ins titute participated after signing theconsent form according to local ethical approval. Through theART (Assis ted Reproductive Technology) procedure, ovarianfollicular cumulus cells (CCs) from the PCOS group (n=۱۲)and healthy (non-PCOS) women (n=۸) were collected duringoocyte retrieval. After total RNA extraction and cDNA synthesis,the expression level of Klotho was quantitatively determinedby Real-Time PCR.Results: Our data indicated a higher Klotho gene expression inCCs of the PCOS group vs. the control group.Conclusion: Current data may imply the correlation betweenupregulation of the klotho gene and its impact on PCOS symptomsdevelopment.

کلیدواژه ها:

Cumulus Cells ، Expression ، Klotho ، PCOS