The Effect of Iron Oxide Nanoparticles on In VitroMaturation of NMRI Mouse Oocyte

Background: Generation of mature oocytes is the main purpose of in vitro maturation (IVM) in order to support futureembryo development. Providing the optimal culture conditionsincluding the use of some antioxidants is necessary to enhanceoocyte developmental competence. Iron oxide nanoparticles(IONPs) due to their antioxidant activities were used to evaluatethe IVM rate of immature oocytes of mice in present s tudy.Materials and Methods: Germinal vesicle (GV) oocytes wereobtained from the ovaries of ۶-۸ weeks NMRI female mice. IVMof oocytes was performed in the presence of IONPs synthesizedby co-precipitation method. To obtain the optimal concentration,different concentrations of nanoparticles (۰.۰۰۱% w/v, ۰.۰۰۲%w/v and ۰.۰۰۴% w/v) in the oocyte culture medium were consideredand the IVM rate was compared with the control group (with۰% w/v of IONPs). In response to the addition of IONPs duringIVM, the survival rate and mitochondrial activity were inves tigatedusing Trypan blue and JC۱ s taining methods, respectively.Results: Using the different concentrations of IONPs showedno significant alteration in nuclear maturity rate of oocytescompared to the control group. Oocyte survival and mitochondrialactivity were decreased significantly (P≤۰.۰۵) by elevateddoses of IONPs compared to the control group.Conclusion: Exposure to IONPs in IVM medium had no undesirableeffects on the oocyte maturation rate. Nevertheless, thetoxicity of IONPs increased in the maximum dose so that anattenuation in mitochondrial activity and oocyte survival wasobserved in the maximum concentration.

کلیدواژه ها:

In Vitro Maturation ، Iron Oxide Nanoparticles ، Oocyte ، Survival Rate