Evaluation of Human Spermatogonia S tem Cell Differentiationon Decellularized Tes ticular Tissue

Background: In males, the division and differentiation of, spermatogonials tem cells (SSCs) cause to the production of gametesand fertility. In vitro culture of SSCs and improvement of culturemethods facilitate the s tudy of spermatogenesis, treatment of infertilitywith male causes and genetic modification through malegametes. In this s tudy, we tried to create similar body conditionsfor the SSCs by culturing the cells on a three-dimensional substrate obtained from decellularized tes ticular tissue.Materials and Methods: After taking the tes ticular tissue fromthe brain dead patient and transferring it to the laboratory, thetes ticular tissue cells were isolated from the tissue by enzymaticmethod. To propagate SSCs, the cells were cultured in a specificculture medium for ۴ weeks. After confirming the identity ofthe SSCs, they were cultured on ۲D and ۳D media with differentialculture medium for ۴ weeks. After this period, the expressionof non-differentiating and differentiating genes in cells ofboth subs trates (two-dimensional and three-dimensional) werecompared by Real-time PCR method.Results: The results showed that the expression of meioticgenes in cells cultured on decellularized tes ticular tissue wassignificantly higher.Conclusion: The results of this s tudy showed that culturings tem cells on the subs trate obtained from decellularized tes ticular tissue can facilitate and improve the process of differentiationof these cells in vitro. This s tudy and similar s tudies couldbe a way to improve infertility in men

کلیدواژه ها:

Decellularization ، Diffetentiation ، SpermatogonialS tem Cells