Cytarabine Effects on Caspase-۳ Gene Expression onBALB/C Mice Ovary Tissue

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 46

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شناسه ملی سند علمی:

RROYAN23_109

تاریخ نمایه سازی: 17 دی 1401

چکیده مقاله:

Background: The increasing use of chemotherapy drugs increasedthe risk of infertility. Apoptosis is a major event in thegerm cell during the Folliculogenesis and Spermatogenesiss tages. Apoptosis occurs during Folliculogenesis and mos t folliclesbecome atresia with apoptosis and a few dominant offollicles will mature. The number of viable germ cells, beingregulated in such a way, is essential for the growth and maturationof germ cells. Caspase-۳, is the major cause of apoptosisand is often used as a marker for the effectiveness of cancertreatment. In the current research the effect of anticancer antimetabolitedrug, Cytarabine, is inves tigated on ovarian tissueand caspase-۳ gene expression.Materials and Methods: Twelve adult female mice of BALB/Cwere divided into a control and tes t groups that received ۱۰۰mg/kg of Cytarabine in the form of IP (Intraperitoneal injection)in single dose. After two weeks, ovarian tissue sectionswere s tained with Hematoxylin-Eosin. Quantitative evaluationof the caspase-۳ gene expression was done with the Real-timePCR method and the data were analyzed with RES t software.Results: All types of follicles decreased which is in the resultof apoptosis increasing. Cytarabine also led to a significant increasein the expression of caspase-۳ gene in ovarian tissue (p< ۰.۰۵).Conclusion: As the increment of Caspase-۳ expression significantlyincreased the apoptosis and subsequently des troyedDNA, RNA, proteins and other cell components the increasedexpression of Caspase-۳ in ovary tissue should be considered inCytarabine application

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نویسندگان

N Amirnahadehkey

Department of Animal Biology, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran

H Mohseni Kouchesfehani

Department of Animal Biology, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran

H Jalali

Department of Animal Biology, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran