Mouse Spermatogonial Cells Differentiation in DecellularizedHuman Placenta Matrix

Background: Natural scaffold production from extracellularmatrix (ECM) components is a promising s trategy for in vitrospermatogenesis.Materials and Methods: The human placenta was decellularizedand the neonatal mouse spermatogonial cells (SCs) werecultured three-dimensionally (۳D) on decellularized placenta.The expression of differentiating markers (Sypc۳, Acrosin andProtamine ۱) was determined after ۳ weeks of culture.Results: The level of Sycp۳ and Prm۱ genes expression wasup-regulated in ۲D and ۳D culture and the highes t level was forcells in the ۳D group. There was an increment in Acrosin geneexpression in the ۳D culture group. The percentage of Prm۱positive cells in the ۳D group (۳۶.۴%) was significantly higherthan in the ۲D group (۱۰.۹۶%) after ۳۵ days of culture.Conclusion: We sugges ted the placental scaffold as a promising bio-scaffold that has high potential for mouse in vitro spermatogenesis

کلیدواژه ها:

Decellularization ، Scaffold ، Placenta ، Spermatogenesis ، Differentiation