Impaired DNA Demethylation in Germ Cells CloselyDepends on Suppressed TET Proteins Expression Level inAn Experimental Varicocele Condition
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
- کد COI اختصاصی: RROYAN23_077
- زبان مقاله: انگلیسی
- تعداد مشاهده: 125
نویسندگان
Department of Basic Sciences, Urmia University, Urmia, Iran
Department of Basic Sciences, Urmia University, Urmia, Iran
Department of Animal Biotechnology, Reproductive BiomedicineResearch Center, Royan Ins titute for Biotechnology, ACECR, Isfahan,Iran
Department of Animal Biotechnology, Reproductive BiomedicineResearch Center, Royan Ins titute for Biotechnology, ACECR, Isfahan,Iran
Department of Animal Biotechnology, Reproductive BiomedicineResearch Center, Royan Ins titute for Biotechnology, ACECR, Isfahan,Iran
چکیده
Background: Failed dynamic DNA methylation and demethylationcollaboratively with a genomic integrity has beenshown to remarkably affect the sperm potential in maintainingmale fertility in varicocele subjects. Ten-Eleven Translocationproteins (۱-۳) (TET ۱-۳) are known to activate the DNA demethylationprocess by oxidizing ۵-methylcytosine (۵mC) inthe pachytene spermatocytes, round and elongated spermatids.The TET enzymes role in pluripotency, differentiation, development,and meiosis is well documented. The current s tudy wasaimed to inves tigate the effect of varicocele on expression levelsof TETs (۱-۳) in the tes ticular tissue.Materials and Methods: For this purpose, ۱۲ male matureWis tar rats were divided into control and varicocele-inducedgroups (n=۶/group). The experimental varicocele was inducedby partially ligating the left renal vein. Following four months,the left tes ticles were dissected out and the mRNA levels ofTET proteins (۱-۳), as well as the TET (۱-۳) + spermatocytesand spermatids, were evaluated by immunohis tochemical s tainingtechniques.Results: In the varicocele-induced group the mRNA levelsof TET ۲, and ۳ were decreased, however, the TET۱ enzymemRNA expression was increased. Moreover, the mean dis tributionsof TET ۱, ۲, ۳+ spermatocytes and spermatids were decreasedin the varicocele-induced group.Conclusion: Minding TET۱ role in regulating pluripotency ofspermatogonia, its high mRNA levels could be related to maintenanceof pluripotency of the s tem cells. However, developingfull spermatogenesis requires all TET enzyme types. Thus,failed spermatogenesis in varicocele conditions could be due tothe lack of TET proteins that leads to impaired DNA methylation/demethylation balance among all germ cell typesکلیدواژه ها
Varicocele, TET Proteins, Spermatogenesis, DNADemethylationاطلاعات بیشتر در مورد COI
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