The Effect of Apigenin Antioxidant on Human SpermQuality after Frozen-Thawed Procedure
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی هیبریدی پزشکی تولید مثل و هجدهمین کنگره هیبریدی فناوری سلولهای بنیادی رویان
- کد COI اختصاصی: RROYAN23_059
- زبان مقاله: انگلیسی
- تعداد مشاهده: 75
نویسندگان
Department of Animal Biotechnology, Reproductive BiomedicineResearch Center, Royan Ins titute for Biotechnology, ACECR,Royan Ins titute, Isfahan, Iran
Department of Animal Biotechnology, Reproductive BiomedicineResearch Center, Royan Ins titute for Biotechnology, ACECR,Royan Ins titute, Isfahan, Iran
Department of Animal Biotechnology, Reproductive BiomedicineResearch Center, Royan Ins titute for Biotechnology, ACECR,Royan Ins titute, Isfahan, Iran.Isfahan Fertility and Infertility Center, Isfahan, Iran
چکیده
Background: The sperm cryopreservation procedure can inducedamaging alterations in sperm function. This process isusually associated with reduced viability and motility, and increasedoxidative s tress and DNA damage in sperm. Althoughseveral mechanisms can be the cause of this damage, the increasein oxidative s tress during freeze-thaw is the mos t importantfactor impairing sperm function. Apigenin is a trihydroxyflavonethat has multiple physiological functions, such asantioxidant, and anti-inflammatory activities. In this s tudy, weaimed to assess the effect of Apigenin on human sperm qualityafter frozen-thawed procedure.Materials and Methods: Following the adminis tration of differentconcentrations of Apigenin during the freezing process(۰.۴, ۰.۲, ۰.۱, ۰.۰۵, ۰.۰ mM), sperm motility, viability, ROSproduction (DCF s taining), lipid peroxidation (Bodipy s taining),and DNA damage (Acridin orange s taining) were assessedon ۱۰ normozoospermic samples before and after the freezing/thawing process. One-Way ANOVA was used for comparisonof s tudy parameters between different concentrations of Apigenin.Results: The analysis of data demons trated that mean percentageof sperm motility and viability significantly were lower after frozen-thawed procedure than before this procedure, andnone of the Apigenin concentrations used in this s tudy couldimprove these two parameters after the freeze-thaw process. Inaddition, we did not observe significant improvement in spermfunction parameters including lipid peroxidation, ROS production,and DNA damage after the use of different concentrationsof Apigenin during freeze-thaw. Only a concentration of ۰.۲mM Apigenin improved sperm DNA damage compared to thecontrol group, which was almos t significant.Conclusion: In this s tudy, we could not observe the antioxidanteffect of Apigenin during the freeze-thaw process on spermfunction. Numerous s tudies using other concentrations of Apigeninon sperm function have been sugges ted to determine theantioxidant properties of Apigenin.کلیدواژه ها
Apigenin, DNA Damage, Freeze-thaw Procedure,Human Sperm, Viability, Motility, Oxidative S tressاطلاعات بیشتر در مورد COI
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