Background: Advanced Glycation End Products (AGEs) are acomplex group of molecules. Endogenous AGEs are producedin our body while exogenous AGEs are consumed through food,smoking and alcohol consumption. AGE could bind receptorsof AGE (RAGE) and this binding has been associated withmany diseases including diabetes, heart disease, kidney failure,Alzheimer’s, as well as aging. AGE and RAGE signalingcould induce lipid peroxidation, reactive oxygen species (ROS)production and trigger inflammation. S tudies have shown thatAGE and RAGE were significantly increased in spermatozoaof diabetic and hyperglycemic men. Since increased oxidative s tress is considered as one of the main causes of male infertilityas well as the final consequence of AGEs diets, we aimed toinduce the AGE model in C۵۷bL mice and assessed the effectof AGE on sperm parameters.Materials and Methods: In this s tudy, ten C۵۷bL male micewere divided into a control and AGE diet groups. After ۳۵ days,all animals were sacrificed, and body weight was assessed.Sperm concentration, motility, and abnormal morphology wereassessed after the extraction of sperm from the caudal left andright epididymis. Sperm concentration, and motility were assessedby CASA sys tem. Sperm morphology was assessed byEosin and Nigrosine s taining. Comparison of body weight, andsperm parameters between two groups were analyzed by independentt tes t. A p-value less than ۰.۰۵ was considered s tatis ticallysignificant.Results: The mean of final body weight and, food intake in theAGE group increased compared to the control group. Unlikethe mean of sperm concentration and morphology which wereno significant differences between the two groups, the mean ofsperm motility was significantly lower in the AGE group thanthe control group (P<۰.۰۵).Conclusion: The result of this s tudy clearly shows that AGEdiets can affect sperm motility more than other parameters. Thediet period with AGE in these mice was about ۳۵ days (a periodof spermatogenesis in mice). It is possible that with increasingAGE diet period, damage to spermatogenesis and sperm functionbecomes more pronounced, which requires further s tudiesin this area.