Albiflorin alleviates DSS-induced ulcerative colitis in mice by reducing inflammation and oxidative stress
- سال انتشار: 1402
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 26، شماره: 1
- کد COI اختصاصی: JR_IJBMS-26-1_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 218
نویسندگان
Department of General Surgery, Bayinguoleng Mongolian Autonomous Prefecture People’s Hospital, Korla, ۸۴۱۰۰۰, Xinjiang, China
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ۲۱۰۰۲۳, Jiangsu, China
Department of General Surgery, Bayinguoleng Mongolian Autonomous Prefecture People’s Hospital, Korla, ۸۴۱۰۰۰, Xinjiang, China
Department of General Surgery, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, ۲۱۵۰۰۹, Jiangsu, China
Department of General Surgery, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, ۲۱۵۰۰۹, Jiangsu, China
چکیده
Objective(s): To clarify therapeutic potential of albiflorin and its intrinsic mechanisms against dextran sulfate sodium (DSS)-induced Ulcerative colitis (UC) mice.Materials and Methods: Sixty male C۵۷BL/۶ mice were randomly divided into five groups: negative control, positive, albiflorin low-dose group, albiflorin high-dose group and treatment control (Salicylazosulfapyridine “SASP”, ۱۰۰ mg/kg) group. Acute colitis was induced in all groups except NC by administration of ۳% DSS for ۷ days. Albiflorin and SASP were administered via the intragastric route twice a day for ۷ days. The changes of colon tissue were assessed by disease activity index (DAI), HE staining, and ELISA. Adrenodoxin expressions of UC colon tissues were evaluated by immunohistochemistry. Western blotting was performed to investigate related protein of the NF-κB and MAPK signaling pathways. Results: It has been found that the albiflorin shares similar influences as the SASP in ameliorating the DSS-induced UC. The reduced DAI and alleviated colon tissue damage were observed in albiflorin intervened groups. Moreover, albiflorin significantly inhibited myeloperoxidase activities and attenuated immuno-inflammatory response and elevated Foxp۳ mRNA in colon tissue. Furthermore, investigations revealed that albiflorin could inhibit adrenodoxin isoform and activate activated phosphorylated NF-κB p۶۵ and IκBα, which consequently suppressed phosphorylated p۳۸ mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK), and c-Jun N-terminal kinase (JNK).Conclusion: These findings showed that albiflorin could alleviate DSS-induced murine colitis by activating inhibiting NF-κB and MAPK signaling pathways. It might be a potential therapeutic reagent for UC treatment.کلیدواژه ها
Albiflorin, Dextran sulfate sodium, MAPK, NF-κB, Ulcerative colitisاطلاعات بیشتر در مورد COI
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