Alteration of immunoregulatory genes expression in mesenchymal stromal cells upon priming with B۱۸R as an interferon binding protein
- سال انتشار: 1402
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 26، شماره: 2
- کد COI اختصاصی: JR_IJBMS-26-2_014
- زبان مقاله: انگلیسی
- تعداد مشاهده: 261
نویسندگان
Immunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran
Stem Cells and Regenerative Medicine Department, Academic Center for Education, Culture, and Research (ACECR)-Khorasan Razavi, Mashhad, Iran
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
Stem Cells and Regenerative Medicine Department, Academic Center for Education, Culture, and Research (ACECR)-Khorasan Razavi, Mashhad, Iran
Immunology Research Center, Inflammation and inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): The B۱۸R protein encoded by the Vaccinia virus decoys Type ۱ interferons and inhibits the activity of several type I IFN members. In vitro transcription protocols benefit from this molecule’s involvement in enhancing cell viability by inhibiting interferon signal transduction. As a result of their immunomodulatory properties and potential to regenerate, mesenchymal stromal cells (MSCs) are increasingly considered an alternative treatment for a wide range of immune disorders. In this study, we investigated the modification of expression of several genes involved in immune-related pathways after preconditioning MSCs with two immune stimuli, including poly(I:C) and LPS. Materials and Methods: ASCs were isolated and primed with B۱۸R, and after exposure to poly(I:C) and LPS, the expression of the same sets of genes as in the previous experiment was evaluated. Following total RNA isolation from primed cells and cDNA preparation, real-time quantitative PCR was performed for several immunomodulatory and immune-related genes, including IDO۱, TDO۲, COX-۲, TGF-β۱, TNF-α, IL-۱β, IL-۶, TLR۳, TLR۴, and MCP-۱. Results: Pretreatment of MSCs with poly(I:C) and LPS significantly increased the expression of all mentioned genes, while upon the B۱۸R challenge followed by poly(I:C) and LPS treatment, they were down-regulated. Finally, it was observed that the relative expression level of IFN-β has significantly decreased in MSCs+B۱۸R+poly(I:C) and LPS in comparison with these groups without B۱۸R.Conclusion: The data indicated that the presence of B۱۸R prevents the overexpression of several immune-related genes, which are overexpressed in the in vitro inflammatory environment.کلیدواژه ها
B۱۸R, Gene expression, Immune-related genes, Interleukin, Lipopolysaccharide, Mesenchymal stromal cells, poly(I:C)اطلاعات بیشتر در مورد COI
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