Restoration of IL-۱۱ and IL-۱۵ cytokine production post calcium modulators and ROS treatment can assist viral clearance both in vitro and in vivo

  • سال انتشار: 1402
  • محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 26، شماره: 2
  • کد COI اختصاصی: JR_IJBMS-26-2_006
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 331
دانلود فایل این مقاله

نویسندگان

Kehkshan Jabeen

Genomics Research Lab, Department of Biological Sciences, International Islamic University Islamabad, Islamabad, Pakistan

Aneela Javed

Healthcare Biotechnology, Atta-ur Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-۱۲ Campus, Islamabad, Pakistan

Asim Waris

School of Mechanical & Manufacturing Engineering (SMME), National University of Sciences and Technology (NUST), H-۱۲ Campus, Islamabad, Pakistan

Shaheen Shahzad

Genomics Research Lab, Department of Biological Sciences, International Islamic University Islamabad, Islamabad, Pakistan

چکیده

Objective(s): Hepatitis B virus (HBV) infection alters the cytokines production to establish persistent infection. A reversion of cytokines back to their normal state can be a promising therapeutic approach to establish an optimal host immune response. Materials and Methods: We investigated the alteration in expression of IL-۱۵ and IL-۱۱ after HBV infection in vitro and in vivo in PBMCs of ۶۳ individuals divided into various HBV-infected patient groups. The mRNA expression was evaluated post-anti-oxidant and calcium modulators treatment by Real-time qPCR. Results: In vitro mRNA expression of both cytokines, post-infection was down-regulated considerably. Interestingly, in line with in vitro results, both cytokines’ in vivo expression was intensively down-regulated in chronic HBV-infected individuals rather than healthy controls. Both cytokines’ expression was up-regulated in cases of recovery compared with the inactive carriers and chronic HBV-infected individuals. IL-۱۵ mRNA expression was significantly up-regulated in both cell lines post EGTA and Ru۳۶۰ treatment while a significant increase was observed in the HepAD۳۸ cell line with NAC and BAPTA treatment. IL-۱۱ mRNA expression was significantly up-regulated in the HepG۲ cell line after all modulator treatments, whereas in the HepAd۳۸ cell line it was observed after BAPTA treatment. Our results thus indicate that viral infection tends to down-regulate the expression of cytokines and an in vivo up-regulation is an indication of recovery. Conclusion: Treatment of anti-oxidants and calcium modulators has resulted in the successful restoration of these cytokines thus pointing towards the use of calcium modulators to boost natural antiviral cytokine production. 

کلیدواژه ها

Hepatitis B virus, Interleukin-۱۱, Interleukin-۱۵, Hepatocellular carcinoma, Reactive Oxygen Species

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.