Beneficial effects of combination therapy with testosterone and hydrogen sulfide by reducing oxidative stress and apoptosis: Rat experimental varicocele model
- سال انتشار: 1401
- محل انتشار: International Journal of Reproductive BioMedicine، دوره: 20، شماره: 11
- کد COI اختصاصی: JR_IJRM-20-11_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 286
نویسندگان
Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
۲. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran.
Department of Physiology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran
چکیده
Background: Despite the effectiveness of testosterone therapy in conditions associated with testosterone deficiency, including varicocele, several dose-dependent side effects limit the clinical use of testosterone therapy. Hydrogen sulfide, a toxic gas in high concentrations but a beneficial molecule in low concentrations, acts as both a major effector and an important inducer of testosterone. Objective: This study investigated whether a subeffective dose of testosterone combined with a subeffective dose of hydrogen sulfide donor sodium hydrosulfide (NaHS) can be effective in an experimental varicocele model through a possible additive effect. Materials and Methods: ۳۰ Wistar rats weighing ۲۰۰-۲۵۰ gr were divided into ۵ groups as (n = ۶/each): sham, varicocele, testosterone (۲۰۰ µg/kg, ۵ times per wk for ۴ consecutive wk), NaHS (۱۵ μmol/L, daily for ۴ consecutive wk) and testosterone + NaHS (۲۰۰ µg/kg, ۵ times per wk + ۱۵ μmol/L, daily, both for ۴ consecutive wk). All animals, except in the sham group, underwent varicocele induction. Results: The coadministration of testosterone and NaHS significantly increased serum testosterone (۱۰.۲۳ ± ۰.۹۵, p = ۰.۰۱), testicular H۲S levels (۶۰۸.۹۴ ± ۲۱.۰۹, p < ۰.۰۰۱), and testicular superoxide dismutase activity (۶۶.۱۴ ± ۱.۵۶, p < ۰.۰۰۱), decreased malondialdehyde levels (۰.۷۷ ± ۰.۵۲, p < ۰.۰۰۱), and B-cell lymphoma ۲-associated X protein to B-cell lymphoma ۲ (۰.۱۶ ± ۰.۰۱, p < ۰.۰۰۱) protein expression ratio in the testicular tissues and improved sperm parameters and testicular histopathology compared to the varicocele group. Conclusion: The combination therapy of subeffective doses of testosterone and NaHS can attenuate the varicocele-induced damages by reducing testicular oxidative stress and apoptosis and thus can be considered an effective approach with fewer side effects.کلیدواژه ها
Apoptosis genes, Hydrogen sulfide, Oxidative stress, Sperm count, Testosterone, Varicocele., ژنهای آپوپتوتیک, سولفید هیدروژن, استرس اکسیداتیو, تعداد اسپرم, تستوسترون, واریکوسل.اطلاعات بیشتر در مورد COI
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