Comparison of Immune Response in Mice Immunized with Recombinant PreS۲/S-C۱۸-۲۷ Protein Derived from Hepatitis B Virus with Commercial Vaccine
- سال انتشار: 1401
- محل انتشار: فصلنامه آسیب شناسی ایران، دوره: 17، شماره: 4
- کد COI اختصاصی: JR_IJP-17-4_009
- زبان مقاله: انگلیسی
- تعداد مشاهده: 238
نویسندگان
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Academy of Medical Sciences of the I.R. of Iran, Tehran, Iran
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
چکیده
Background & Objective: The vaccine available to prevent Hepatitis B virus disease is ineffective in ۵% of people due to the use of HBsAg as a weak immunogen. In the present study, PreS۲/S fused to C۱۸-۲۷ peptide fragment as an effective antigen and is proposed as a promising vaccine candidate compared with the conventional vaccine prescribed in the vaccination program.Methods: After the synthesis of PreS۲/S genes and C۱۸-۲۷ peptide fragment in pET۲۸a, the recombinant protein was confirmed by Western blotting. The efficacy of the PreS۲/S-C۱۸-۲۷ protein was compared with the conventional vaccine injected into five groups of rats. Finally, the cytokine level of IF-r, IL-۲, IL-۴, IL-۱۰, TNF-a, IgG۱, and IgG۲a were measured using the ELISA method.Results: This study showed no significant difference between the recombinant vaccine group and PBS control group in the IF-r test, but there was a significant difference between groups testing IL-۲ and IL-۱۰. In addition, the group receiving the recombinant vaccine with CPG adjuvant at a dilution of ۱/۱۰ in the IgG total test on days ۱۴ and ۴۵ after the first injection showed a significant difference in comparison with other groups.Conclusion: This study showed no statistically significant difference between the recombinant protein vaccine group and the conventional vaccine group. The Th۱- mediated immune responses obtained from recombinant proteins with and without CPG performed better than conventional vaccines, possibly due to the functional deficiency of the available vaccines.کلیدواژه ها
CpG ۷۹۰۹, Hepatitis B Core Antigen, Hepatitis B Vaccines, Hepatitis B virus, preS۲HBsAgاطلاعات بیشتر در مورد COI
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