The study of P-glycoprotein A, G-glutamylcysteine synthetase ۱, and aquaglyceroporin ۱ genes expression innon-healing zoonotic cutaneous leishmaniasis cases

Background and aims: Regarding the increasing numbers of clinical antimonial-resistant Leishmaniasis, understanding the reasons fordrug resistance is helpful. This study aimed to find the expression level of the genes related to resistance, P-glycoprotein A (PgpA),G-glutamylcysteine synthetase ۱ (Gsh۱), and aquaglyceroporin ۱ (Aqp۱) in antimonial-resistant clinical isolates.Methods: Samples were isolated from leishmaniasis ulcers of ۱۰ non-healing patients and the species were identified by the nestedpolymerasechain reaction (PCR) method. In vitro experiments were performed using the amastigote-macrophage model by J۷۷۴ cellline, and in vivo studies were conducted by animal model, the Balb/c mice. Finally, the values of genes expression were determined byquantitative-reverse transcription (q-RT) real-time PCR method and then compared with non-resistant Leishmania major (MRHO/IR/۷۵/ER).Results: Molecular identification showed that all isolated protozoa were L. major. The isolated samples from clinical resistant patientsrepresented no increase in expression in the tested resistance genes (P > ۰.۰۵). Finally, it was found that the lack of improvements inpatients was not associated with the increased expression of resistance genes.Conclusion: In general, no inherent resistance was observed in the tested samples neither a correlation between the healing of lesions andthe level of genes expression.

کلیدواژه ها:

Drug resistance ، Gene expression ، Zoonotic cutaneous leishmaniasis ، q-RT PCR