Regulatory Effects of Thymoquinone on Dopamine Level in Neuronal Cells Exposed to Amphetamine: an in Vitro Study

  • سال انتشار: 1399
  • محل انتشار: مجله تحقیقات بیهوشی سلولی و مولکولی، دوره: 5، شماره: 4
  • کد COI اختصاصی: JR_SBMU-5-4_013
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 120
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نویسندگان

Nurul Farah Aina MD Fauzi

Faculty of Medicine, University of Sultan Zainal Abidin (UniSZA), ۲۰۴۰۰ City Campus Kuala Terengganu, Terengganu Darul Iman, Malaysia

Nor Hidayah Abu Bkar

Faculty of Medicine, University of Sultan Zainal Abidin (UniSZA), ۲۰۴۰۰ City Campus Kuala Terengganu, Terengganu Darul Iman, Malaysia

Nasir Mohamad

Faculty of Medicine, University of Sultan Zainal Abidin (UniSZA), ۲۰۴۰۰ City Campus Kuala Terengganu, Terengganu Darul Iman, Malaysia

Liyana Hazwani Mohd Adnan

Faculty of Medicine, University of Sultan Zainal Abidin (UniSZA), ۲۰۴۰۰ City Campus Kuala Terengganu, Terengganu Darul Iman, Malaysia

Nor Suliana Mustafa

Faculty of Medicine, University of Sultan Zainal Abidin (UniSZA), ۲۰۴۰۰ City Campus Kuala Terengganu, Terengganu Darul Iman, Malaysia

Nor Zidah Ahmad

Faculty of Medicine, University of Sultan Zainal Abidin (UniSZA), ۲۰۴۰۰ City Campus Kuala Terengganu, Terengganu Darul Iman, Malaysia

چکیده

Background: Amphetamine (AT) is a potent central nervous system stimulant that is capable of producing damaging effects to the central dopaminergic pathway; used both for medical purposes and also abused recreationally. One of the potential naturally occurring compounds is thymoquinone (TQ), an active compound of Nigella sativa; which is known for its cellular protective effects. The objectives of this study were to determine the IC۵۰ values of AT and TQ on differentiated SH-SY۵Y neuronal cells and to evaluate the changes of dopamine (DA) level in the cells exposed to AT after co-administering with TQ.Materials and Methods: Differentiated SH-SY۵Y cells were grown in a cell culture flask containing DMEM/F۱۲ medium supplemented with ۱۰% (v/v) fetal bovine serum and ۱% (v/v) penicillin/streptomycin. The IC۵۰ value of TQ and AT in differentiated SH-SY۵Y cells was determined by using ۳-(۴,۵-dimethylthiazol-۲-yl)-۲,۵-diphenyl tetrazolium bromide (MTT) assay. The DA level was determined by using the Enzyme-Linked Immunosorbent Assay (ELISA) kit.Results: The IC۵۰ values of AT and TQ were ۱۵۹۶ μM and ۹۲۶ μM, respectively. Co-administration of ۴۰ μM of AT and ۳۰ μM of TQ demonstrated a significant increase in DA level at ۴۸ hours of exposure when compared to the administration of AT group (P≤۰.۰۵).Conclusion: These findings suggested that TQ has a role in maintaining the DA activity after long-term AT exposure.

کلیدواژه ها

amphetamine, thymoquinone, differentiated SH-SY۵Y cells, dopamine, lactate dehydrogenase activity

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