Evaluation of the Virulence Genes in Quinolone and Fluoroquinolones- resistant Uropathogenic Escherichia coli Isolates
- سال انتشار: 1401
- محل انتشار: فصلنامه میکروب شناسی پزشکی ایران، دوره: 16، شماره: 6
- کد COI اختصاصی: JR_IJMM-16-6_010
- زبان مقاله: انگلیسی
- تعداد مشاهده: 150
نویسندگان
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Pharmacology, Hamadan University of Medical Sciences, Hamadan, Iran
Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Biological Sciences, National University of Medical Sciences (NUMS), Rawalpindi, Pakistan
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
چکیده
Background and Aim: Uropathogenic Escherichia coli (UPEC) is the most prevalent causative agent of urinary tract infections (UTIs) in both community and hospital settings. Annually about ۱۵۰ million people globally develop UTIs, resulting in increased healthcare costs. The current study examined the identification and the frequency distribution of virulence factors among fluoroquinolones-resistant (FQs-R) and fluoroquinolones-susceptible (FQs-S) UPEC strains in Hamadan hospitals, west of Iran. Materials and Methods: One hundred-seventy urine samples were collected consecutively from inpatients at three hospitals in Hamadan from March to September ۲۰۱۸. The UPEC isolates were identified using biochemical tests and polymerase chain reaction (PCR). The disk diffusion and the broth microdilution methods determined the antimicrobial susceptibility and the minimum inhibitory concentration (MIC) of Ciprofloxacin. The multiplex-PCR method investigated the prevalence of pap, aer, and hly genes. Results: Among ۱۷۰ urine samples collected from inpatients, E. coli was the most common isolate, with a frequency of ۱۲۵ (۷۳.۵%). Resistance to Nalidixic acid and fluoroquinolones, including Ciprofloxacin, Norfloxacin, and Ofloxacin, was detected in ۸۸.۸%, ۷۱.۲%, ۷۰.۴%, and ۶۸.۸% of UPEC isolates, respectively. The prevalence of hly and pap genes in FQs-R strains was significantly lower than in FQs-S strains. Conclusion: The high-level antibiotic resistance to quinolones & fluoroquinolones and heterogeneity of virulence genes among clinical UPEC isolates need strong attention.کلیدواژه ها
Uropathogenic Escherichia coli, Virulence Factors, Quinolone-Resistant, Fluoroquinolone-Resistant, اشرشیاکلی اروپاتوژنیک, فاکتورهای ویرولانس, مقاوم به کینولون, مقاوم به فلوروکینولوناطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.