Phylogeny and genetic diversity of Fusarium graminearum species complex associated with Fusarium head blight of wheat in Moghan plain (Iran)

Thirty-seven isolates of Fusarium graminearum species complexobtained from wheat heads with Fusarium head blight symptoms were selected and used for phylogenetic studies. They were collected from different localities of Moghan plain (Ardebil province, Iran). Partial sequences of translation elongation factor ۱-alpha (TEF), putative reductase (RED) and UTP-ammonia ligase (URA) genes were amplified using specific primers and sequences were analyzed using Maximum Parsimony method. Almost all strains of F. graminearum species complex belonged to F. graminearum sensu stricto. The results indicated homogeneity within F. graminearum species complex. Inter-simple sequence repeat markers (ISSR) were employed to study the genetic structure of F. graminearums.s. populations collected from five localities in Moghan plain. The ISSR markers were generated by five primers and data analysed using UPGMA method with Jaccard's coefficient. Cluster analysis showed that all isolates were divided into four clades with ۸۲% similarity level. The analysis of molecular variation indicated that most of the gene diversity (۹۷%) was distributed within populations, whereas ۳% of the variation was found among populations. Phylogenetic species identification and genetic diversity knowledge of major agent of FHB disease will be useful in defining the risk of pathogen evolution as well as benefiting disease management strategies.

کلیدواژه ها:

Microsatellite markers ، RED ، TEF ، URA ، wheat scab