Investigation of phylogenetic relationships and genetic diversity of Seidlitzia rosmarinus populations by ISSR marker in some regions of Iran
- سال انتشار: 1400
- محل انتشار: دوفصلنامه رستنیها، دوره: 22، شماره: 1
- کد COI اختصاصی: JR_ROST-22-1_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 138
نویسندگان
PhD Student, Science and Research Branch, Islamic Azad University, Tehran, Iran
Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Associate Prof., Department of Biology, Central Tehran Branch, Slamic Azad University, Tehran, Iran
Associate Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده
The genus Seidlitzia has four herbaceous species viz. S. florida (M.B.) Bge. ex Boiss., S. cinerea (Moq.) Bge. ex Botsch. and S. stokii that are herbaceous, and S. rosmarinus (Ehrenb.) Bge. ex Boiss. is shrubby belong to the family Amaranthaceae. In the present study, ISSR molecular markers were used to evaluate the genetic diversity among ۸۵ plant samples from ۱۷ populations of S. rosmarinus. Four random primers were used to perform ISSR reactions and in total ۲۲۱ DNA bands were obtained. After forming ۰ and ۱ matrix with the help of GenAlex and NTSYS software, it was used to interpret ISSR analysis data. Molecular analysis of variance (AMOVA) was performed to estimate genetic diversity out of ۸۵ individuals. Of total diversity, the highest amount of genetic diversity (۶۸%) was related to intra-population diversity, ۲۸% of inter-population diversity, and ۴% of inter areas was calculated. MCMC analysis using Structure ۲.۳.۴ software was used to investigate the genetic diversity populations of S. rosmarinus. Main coordinates analysis by the PCOA method was also used for extract maximum information from molecular data.کلیدواژه ها
Amaranthaceae, cluster analysis, molecular analysis of variance, UPGMA methodاطلاعات بیشتر در مورد COI
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