Experimental Protocol for Detecting SARS-CoV-۲ in Screenings and Grit Samples of Wastewater Treatment Plants
- سال انتشار: 1401
- محل انتشار: فصلنامه بهداشت محیط و توسعه پایدار، دوره: 7، شماره: 3
- کد COI اختصاصی: JR_JEHSD-7-3_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 217
نویسندگان
Department of Environmental Health Engineering, Maragheh University of Medical Sciences, Maragheh, Iran.
Department of Environmental Health Engineering, Maragheh University of Medical Sciences, Maragheh, Iran.
Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.
Department of Environmental Health Engineering, Maragheh University of Medical Sciences, Maragheh, Iran.
Office of Waste Management Organization, Maragheh Municipality, Maragheh, Iran.
Department of Environmental Health Engineering, Maragheh University of Medical Sciences, Maragheh, Iran.
چکیده
Introduction: Although various liquid, solid, and gaseous streams of wastewater treatment plants (WWTPs) have been analyzed in many studies for the presence of SARS-CoV-۲ RNA, no study was found to sample and detect SARS-CoV-۲ RNA in screenings and grit samples separated from primary treatment units of WWTP. Hence, this study aims to provide an experimental protocol for sampling and extracting SARS-CoV-۲ RNA from screenings and grits separated from WWTPs. Materials and Methods: First, sampling was conducted to extract SARS-CoV-۲ RNA from screenings and grit samples. After sample processing and viral RNA extraction, SARS-CoV-۲ RNA detection was performed by one-step reverse transcription quantitative polymerase chain reaction (RT-qPCR). Results: Based on the results, SARS-CoV-۲ RNA was successfully extracted from screenings and grit samples of the studied WWTP with concentrations of (۱.۵۴ –۳.۹ × ۱۰۴) and (۰.۸ – ۲.۳ × ۱۰۴) genomic copies/L, respectively. Conclusion: Considering the successfully isolation and detection of SARS-CoV-۲ viral RNA in solid phase samples of WWTP, this method can be applied for extracting SARS-CoV-۲ RNA and maybe other viruses from the screenings and grit samples of WWTPs in related studies.کلیدواژه ها
SARS-CoV-۲, Waste Water, Mass Screening.اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.