Isolation and molecular identification of two rutin-producingendophytic fungi from Caper (Capparis spinosa L.)

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 42

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شناسه ملی سند علمی:

MEDISM23_628

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Flavonoids are a group of polyphenolic compounds that are naturallyfound in plants and many flavonoids are the basis of herbal medicines and have effectivepharmacological effects. Rutin is one of the flavonoids that is used as a reagent in the study offlavonoids and is also considered an antioxidant. This study was conducted to isolate and identifyrutin-producing endophytic fungi from C. spinosa.Methods : Sampling was performed in ۱۰ regions of Iran and was taken from different tissues(including leaves, stems, roots, and fruits). After surface disinfection, explants were placed onPDA medium and the fungal isolates were purified. Molecular identification of isolates wasperformed by sequencing ITS۱-۵.۸S-ITS۲. Morphological identification of fungi was performedby microscopic slide preparation and fungus identification keys. Methanolic extract of fungi wasprepared by the maceration method and the presence of rutin was investigated using the HPLCtechniqueResults : A total of ۲۴ fungi were identified. HPLC analysis showed that the peak of standardflavonoids including rutin, quercetin, and apigenin appeared at ۳.۸۲, ۷.۶۰, and ۱۲.۴۰ min,respectively. Results showed that only two isolates were able to produce rutin. A comparison ofthe mean production of rutin showed a significant difference between the two fungi. Alternariaalternata M۲۸ (۲۲.۹۴ ppm) had the highest amount of rutin production. Results also showed thatrutin production in the mycelial tissue was more than in the extracellular medium so that in A.alternata M۲۸ and Paecilomyces maximus had rutin content of about ۶۷.۵ and ۵.۵۶ times,respectively.Conclusion : Fungal rutin in methanolic extracts was characterized by HPLC. This is the firstreport that endophytic fungi have an acceptable potential for rutin production from C. spinosa andmore research on these isolates can increase the amount of rutin production in them and lead tocommercialization.

کلیدواژه ها:

نویسندگان

Melika Esfandiari

Department of Microbiology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran.

Mohsen Rajabi

Department of Horticulture, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

Mohammad Reza Azimi Moghadam

Department of Horticulture, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

Ali azizi

Department of Horticulture, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

Jalal Soltani

Department of Phytopathology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.