Phyto-Mediated Silver Nanoparticles for AntibacterialPerformances by Salicornia Extract

Background and Aim : Salicornia is a genus of succulent, halophytic (salt tolerant) floweringplants in the family Amaranthaceae that grow in salt marshes and on beaches. In this project, silvernanoparticle was synthesized using Salicornia leaves extract as a reducing and capping agent bymicrowave irradiation method for Antibacterial Performances.Methods : In this project, phytosynthesis of stable silver nanoparticles was done using Salicorniaextract. These phytosynthesized nanoparticles were characterized with the help of UV–Visspectrophotometer, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), andTransmission electron microscopy (TEM). Later, their antibacterial activity was screened againstboth gram-negative and gram-positive microorganisms by MIC and MBC method.Results : The formation of silver nanoparticles was confirmed by Surface Plasmon Resonance(SPR) as determined by UV-Vis spectra at ۴۲۵ nm. Fourier transform infrared spectroscopy showsthat the functional groups are carboxyl, amine, and phenolic compounds of seed extract which areinvolved in the reduction of silver ions. The XRD peaks at ۳۸?C, ۴۴?C, ۶۴?C and ۷۷?C can beindexed to the (۱ ۱ ۱), (۲ ۰ ۰), (۲ ۲ ۰) and (۳ ۱ ۱) Bragg’s reflections of cubic structure of metallicsilver, respectively. Silver nanoparticles of size ranging from ۲۵-۹۰ nm of spherical shape werecharacterized using transmission electron microscopy (TEM). The synthesized Ag-NPs exhibitedgood antibacterial potential against gram positive and gram negative bacterial strains.Conclusion : Thus, this method can be used for rapid and ecofriendly phyto-synthesis of stableAg nanoparticles of size range ۲۵–۹۰ nm possessing antibacterial activity suggesting their possibleapplication in medical industry.

کلیدواژه ها:

Salicornia ، Silver nanoparticles ، Antibacterial Activity ، MBC ، TEM ، MedicalIndustry.