Evaluation of Antibacterial Combination Activities of Honeyand Alcoholic Extract of Crocus sativus Plant on Staphylococcus aureus:An in vitro Study

Background and Aim : Bacterial resistance to antibiotics is one of the world most urgent publichealth and development threat in this century. Accordingly, search for new drugs continues. Today,medicinal plants are known as a source of antimicrobial agents. The purpose of this study is toevaluate the antibacterial combination activities of honey and alcoholic extract Crocus sativus onStaphylococcus aureus by in vitro experiments.Methods : We prepared the alcoholic extract of Crocus sativus plant and lyophilized the plantextract and honey. Then, the antibacterial activities of these two compounds were examinedseparately and the combination of honey with alcoholic extract of Crocus sativus plant,respectively with proportions of (۵۰%, ۵۰%), (۷۵%, ۲۵%), (۲۵%, ۷۵%) was determined by theminimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) againstreference bacterial strain in accordance with the Clinical and Laboratory Standards Institute(CLSI) guideline.Results : Crocus sativus exhibited more potent antibacterial activities against Staphylococcusaureus rather than compounds that we determined. Geometric means of MIC for Crocus sativuswere recorded at ۱ mg/μL (Staphylococcus aureus).Conclusion : Due to the equality of MIC and MBC results of Crocus sativus alcoholic plantextracts and also because of the presence of active antibacterial substances in this plant, alcoholicextract of Crocus sativus plant can be a good substitute for the treatment of infections caused byStaphylococcus aureus.

کلیدواژه ها:

Antibacterial activities ، Crocus sativus ، Staphylococcus aureus ، in vitro