An Evaluation of Antibacterial Effects of Human AmnioticFluid on Pathogenic and Probiotic Bacteria in Vitro
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_517
- زبان مقاله: انگلیسی
- تعداد مشاهده: 243
نویسندگان
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
Department of Gynecology and Obstetrics, Medical Faculty, Birjand University of Medical Sciences, Birjand, Iran
Medical Toxicology and Drug Abuse Research Center, Birjand University of Medical Sciences, Birjand, Iran
Medical Toxicology and Drug Abuse Research Center (MTDRC), Birjand University of Medical Sciences, Birjand, Iran
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran.
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran.
چکیده
Background and Aim : Introduction: It is proposed that some compounds in amniotic fluid causethe fetus to remain free from microbial contaminations. This study aimed to evaluate theantibacterial effects of amniotic fluid and to compare its effects on pathogenic and probioticbacteria.Methods : Method: This experimental study was conducted on amniotic fluid obtained from ۴۳healthy mothers who gave birth by selective cesarean section. The samples transferred into thelaboratory under sterile condition to investigate the antibacterial effects of amniotic fluids on ۵standard bacterial strains, including Bacillus cereus, Staphylococcus aureus, Pseudomonasaeruginosa, Streptococcus pyogenes and Lactobacillus plantarum by agar well-diffusion method.The inhibition zone diameter of each cultured bacteria around the wells filled with amniotic fluidsample was measured after an overnight incubation at ۳۷º C. Data analysis was performed by SPSSsoftware, version ۲۲ at a significance level of ۰.۰۵ by Kruskal-Wallis and Mann-Whitney tests.Results : Findings: Amniotic fluid revealed an inhibitory effect on the growth of bacterial strainsto a different extent. Staphylococcus aureus and Streptococcus pyogenes strains showed growthinhibition in ۳۹% and ۱۷% of samples, respectively. In other bacterial strains, there was growthinhibition in less than ۵% of the samples. Also, the zone of growth inhibition for Staphylococcusaureus was significantly higher than the other strains (p-value < ۰.۰۰۱) and the zone of growthinhibition for Streptococcus pyogenes was also significantly higher than Lactobacillus plantarum(p-value = ۰.۰۰۴), Bacillus cereus (p-value = ۰.۰۰۴) and Pseudomonas aeruginosa (p-value =۰.۰۰۱) strains.Conclusion : Conclusion: The results of this study demonstrate antibacterial activity of amnioticfluid on pathogenic bacteria but to a different extent. Our findings also suggest that antibacterialeffect of amniotic fluid on pathogenic bacteria is significantly higher than the probiotic one.کلیدواژه ها
Amniotic fluid, Antibacterial, Pathogenic bacteria, Probiotic bacteriaمقالات مرتبط جدید
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