Investigated the therapeutic effect of Tilapia Piscidin ۴ (TP۴)peptide on antibiotic resistance nosocomial pathogens in vitro and invivo

Background and Aim : Antimicrobial peptides (AMPs) are considered potential agents to replaceconventional antibiotics. Tilapia piscidin ۴ (TP۴) is a peptide with antimicrobial activity. Thisstudy investigated the potential cytotoxicity and hemolytic activity of TP۴ as well as its therapeuticefficacy in vitro and in vivo on nosocomial pathogens resistant to conventional antibiotics.Methods : Cytotoxicity and hemolytic activity of TP۴ were assessed in the human primaryfibroblast cell line (C۶۵۴) and human red blood cells (RBCs), respectively. BALB/c mice wereused to determine the median lethal dose (LD۵۰) and a sub-lethal dose of TP۴ administeredintraperitoneally (i.p.). The antibacterial activity of TP۴ was assessed against extensively drugresistant(XDR) Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus (MRSA),and KPC-producing Klebsiella pneumonia (KPC-KP) in vitro and in vivo.Results : TP۴ exhibited no cytotoxicity against the human primary fibroblast cell line (IC۵۰=۱.۹۱g/ml) at MIC and MBC values ranging from ۴ to ۲۵.۶ g/ml. Moreover, in vivo data indicated thatsub-lethal doses of TP۴ (۰.۸ mg/kg, i.p.) had minimal cytotoxicity for the liver or kidney and wereunable to completely alleviate the peritoneal load of antibiotic-resistant bacteria in experimentalmodels.Conclusion : Despite the promising in vitro results, this study's in vivo findings indicate that asub-lethal dose of TP۴ was unable to induce a perfect cure in mice infected with antibiotic-resistantnosocomial pathogens.

کلیدواژه ها:

Antimicrobial peptides ، Tilapia piscidin ۴ ، TP۴ ، Antibiotic resistance