Immunogenicity of the Recombinant Cryptococcus neoformansHSP۷۰, a potential Candidate for Developing an ELISA kit

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 42

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شناسه ملی سند علمی:

MEDISM23_505

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Cryptococcus neoformans is an encapsulated fungal pathogen that causeslife-threatening meningoencephalitis in immunocompromised patients. This yeast secretes severalpotent immunogenic proteins by secretory vesicular mechanisms, such as HSP۷۰ chaperone.Methods : The PCR-amplified HSP۷۰ gene was cloned into a PET-۲۸a(+) expression vector. Thepurified recombinant HSP۷۰ (rHSP۷۰) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit wastested against the whole lysate of C. neoformans in ELISAResults : The antibodies in the rabbit’s serum recognized lysate of C. neoformans yeast. Thehighest antibody levels were achieved after the third booster injection.Conclusion : The rHSP۷۰ showed to be a reliable candidate for the designing and development ofan ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens

نویسندگان

Pooya Jafari

Medical Student, Research Commite, Qom University of Medical Sciences, Qom, Iran

Roohollah Fateh

Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran