Zohre Ahmadi - Faculty of Science, Karaj Azad University, Karaj, Iran
Nader mosavari - Reference Laboratory for Bovine Tuberculosis, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization, Tehran, Iran.
Mostafa ghaderi - Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.

Background and Aim : background and aims: Due to the importance of tuberculosis in humansocieties and its role in transmission to humans and the lack of examination of the presence ofmycobacterial contamination in the wildlife of Alborz province, the aim of this pioneering researchis to isolate and determine the molecular identity of mycobacterium from zoos in Alborz province.Methods : Materials and methods: This study was carried out on animal feces samples from zoosin Alborz province, and they were separated by NAOH and HPLC methods. The research andanalysis was carried out using culture and preparation of Nelson staining slides, PCR and PCRRFLP.Results : Findings: In this study, pcr۱۶srRNA was extracted from DNA samples to confirm thegenus of Mycobacterium and Multiple rounds of ۶۱۱۰ PCR PCR IS۹۰۰ and PCR IS۹۰۱ testsshowed paratuberculosis and other non-tuberculosis species.Conclusion : Conclusion: Considering the importance of increasing diseases caused by nontuberculousmycobacteria all over the world, a study was conducted to evaluate the frequency ofthese diseases in Iran in order to identify and investigate the prevalence of non-tuberculousmycobacteria in Iran from different clinical samples, during a An ۸-year period was conducted inMasih Daneshvari Hospital. In this descriptive study, patients with pulmonary tuberculosissymptoms were conducted during ۱۹۸۳-۲۰۱۹ and are isolated from environmental samples,considering the role of some mycobacterial species as a reservoir and their role in transmission tohumans and the lack of examination of the existence of mycobacterial contamination in the wildlifeof Alborz province, the necessity of investigation in this field seems necessary. Although manyresearches have been done on wildlife animals in the world, but not many studies have been donein Iran

Mycobacterium,pcr RFLP,Molecular identification,isolated