Detection of rifampicin resistance strains of Mycobacteriumtuberculosis using multiplex allele-specific polymerase chain reaction(MAS-PCR) in Ardabil, Iran

Background and Aim : Mycobacterium tuberculosis (MTB) is the main causative agent of ahighly infectious disease called tuberculosis (TB). Currently, TB remains a major public healthconcern worldwide and is one of the most common life-threatening infections due to the high ratesof multidrug-resistant tuberculosis (MDR-TB) and drug-resistant tuberculosis (DR-TB) cases inmany parts of the world. Multidrug-resistant tuberculosis (MDR-TB) is defined as strains that areresistant to the first line of anti-tuberculosis agents, including Rifampin (RIF) and Isoniazid (INH),two most potent anti-TB drugs. Resistance to Rifampin alone is an identification marker of MDRstrain. Therefore, this study was conducted to determine the rate of rifampicin-resistant M.tuberculosis (RR-MTB) among presumptive TB patients attending the Health Center, Ardabil,Iran.Methods : This cross-sectional study was conducted in the Health Center of Ardabil province from۲۰۱۶ to ۲۰۲۰. After checking the completeness of the necessary information on tuberculosispresumptivecases, a lot of ۱۱۱ sputum and bronchoalveolar lavage (BAL) samples were collectedfrom Registration booklets of the health center of Ardabil province.Results : Among ۱۱۱ rifampicin-susceptible isolates, ۳۶ (۳۲.۴۳%) rifampicin-resistancetuberculosis isolates were identified. The frequency of mutations was in codon ۵۲۶ (۲۶%), codon۵۱۶ (۱۱.۷۱%), and codon ۵۳۱ (۷.۲۰%) in the rpoB gene, respectively. In our study, the mostcommon mutation was reported in codon ۵۲۶.Conclusion : This study showed that in the samples isolated from Ardabil city, the high rate ofmutation associated with rifampin-resistant tuberculosis strains was located in the rpoB۵۲۶ gene.This result confirms that the screening of this gene region is suitable for determining resistance torifampin in clinical samples of M. tuberculosis in Ardabil city. Molecular methods are appropriateand rapid to identify the MDR strain M. tuberculosis.

کلیدواژه ها:

Mycobacterium tuberculosis ، Drug resistance ، rpoB gene ، tuberculosis (TB) ، rifampicin (RMP) ، multidrug drug-resistant tuberculosis (MDR).