A case report of feline tuberculosis: diagnosis and its dangersto humans

Background and Aim : The possibility of feline tuberculosis is increasingly recognized as azoonotic disease. The treatment is challenging, and the prognosis varies greatly from case to case.Tehran Oxygen Veterinary Hospital received a Cat with two years of age with symptoms ofanorexia, weight loss, shortness of breath, and coughing. As a result of physical examination, thepatient showed tachypnea (respiratory rate ۴۰ bpm) and increased inspiratory and expiratory effort,as well as mild lymphadenomegaly of the mandible and harsh lung sounds. Eventually, the catdied from bloody diarrhea after two days.Methods : To determine the cause of death, an autopsy was performed. For smear preparation,culture, and molecular testing, samples were taken from granulomatous lesions. A Ziehl-Neelsenstaining was Conducted. In addition, Lowenstein-Jensen (LJ) Medium (contains sodium pyruvate)was used for culture. A ۳۷°C incubator was used for ۸ consecutive weeks of incubation. Based onstandard protocols, a number of biochemical tests were performed, including catalase production,niacin production, and nitrate reduction. Van Soolingen method was used to extract DNA. PCRwas performed using JB۲۱-JB۲۲ specific primers. As a positive control, a standard strain ofMycobacterium bovis (ATCC/۱۹۲۱۰) was used.Results : Miliary tuberculosis was observed on autopsy, especially in the lungs. Multifocalgranulomatous pneumonia were clear and palpable, the intestines were hyperemic, and the kidneyscontained Multifocal granulomatous nephritis. In smear samples taken from tuberculosis, acid-fasttuberculosis bacilli were present. Biochemical tests confirmed M. bovis. based on culture results,tuberculosis was confirmed and the PCR test detected M. bovis species.Conclusion : As a member of the Mycobacterium tuberculosis complex (MTBC), M. bovis causestuberculosis in humans, cattle, deer, dogs, and cats. It affects a wide range of hosts and is one ofthe most important zoonotic hazards. In cats, it is usually caused by ingestion of infected meat,unpasteurized milk, wild rodents or contact with contaminated material. this report and theobservation of this pathogen illustrate the critical role meat inspection plays in ensuring foodsafety, and they raise the need for additional research into infection mechanisms and immuneresponses of cats to M. bovis.

کلیدواژه ها:

tuberculosis ، Mycobacterium bovis ، Autopsy ، Case report ، Miliary tuberculosis ، Bacteriology