The control of Multi-Drug Resistant Klebsiella pneumoniaewound infection by Bacteriophage in a Rat Model
محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 80
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شناسه ملی سند علمی:
MEDISM23_448
تاریخ نمایه سازی: 16 مهر 1401
چکیده مقاله:
Background and Aim : Multi-drug-resistant Klebsiella pneumoniae (MDR-KP) has persistentlyraised beyond antibiotic control. Wound infection kills many patients yearly due to the entry ofmultidrug-resistant (MDR) bacterial pathogens into the skin gaps. However, a bacteriophage(phage) is a potential antibiotic alternative for treating bacterial infections. The aims of study toisolate and characterize a specific phage and evaluate its topical activity against MDR-KP isolatedfrom infected wounds.Methods : A lytic phage KshKPC-M was isolated using a clinical isolate KP(Ksh-۱) as a hoststrain and then characterized. Additionally, the phage was assessed for its in vitro host range,temperature, ultraviolet (UV), and pH sensitivity. The therapeutic efficiency of phage suspensionand a phage-impeded gel vehicle were assessed in vivo against a K. pneumoniae infected woundon a rat model.Results : The phage produced a clear plaque and was classified as Siphoviridae. The phageinhibited KP(Ksh-۱) growth in vitro in a dose-dependent pattern, and it was found to resist hightemperature (<۷۰ ◦C) and was primarily active at pH ۵; moreover, it showed UV stability for ۴۵min. Phage-treated K. pneumoniae inoculated wounds showed the highest healing efficiency bylowering the infection. The quality of the regenerated skin was evidenced via histologicalexamination compared to the untreated control group.Conclusion : This research represents the evidence of effective phage therapy against MDR-KP.
کلیدواژه ها:
نویسندگان
Mehrdad Mohammadi
Department of Medical Microbiology and Immunlogy, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
Seyed Davar Siadat
Department of Mycobacteriology and Pulmonary Research and Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran
Mahmoud Saffari
Department of Medical Microbiology and Immunlogy, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran