Investigating on the biological interaction of maize seedlingsoft rot bacteria
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_430
- زبان مقاله: انگلیسی
- تعداد مشاهده: 143
نویسندگان
M.Sc. Student of Plant Pathology, Department of Plant Protection, Agriculture Faculty, Shahid Chamran University of Ahvaz, Iran.
Assistant Professor of Plant Pathology, Department of Plant Protection, Agriculture Faculty, Shahid Chamran University of Ahvaz, Iran.
Assistant Professor of Plant Pathology, Department of Plant Protection, College of Agriculture, University of Tehran, Karaj, Iran.
چکیده
Background and Aim : In recent decades, bacteriocins have received substantial attention asantimicrobial compounds. Infections caused by antibiotic-resistant bacteria have been declared asa global threat to public health. Bacteriocins represent a potential solution to this worldwide threatdue to their broad or narrow-spectrum activity against antibiotic-resistant bacteria. In the presentstudy, the capacity of eight bacterial species causing the soft rot disease of maize to producebacteriocin were investigated.Methods : During the years ۲۰۲۰-۲۰۲۱, eight bacterial species causing stem and maize crown softrot of were isolated and identified from Tehran, Alborz and Khuzestan Provinces. Inoculationswere made by swabbing approximately ۱۰ μl of bacterial suspension (۱ × ۱۰۸ CFU/mL) on thesurface of yeast extract peptone glucose agar (YPGA) medium in three corners of an imaginarytriangle; the cultures were then incubated at ۲۵–۲۷ °C for ۷۲ h. Approximately ۲۰۰ μl of theresulting suspension of the strains was sprayed the surface of the YPGA plates, containingpreviously inoculated isolates.The plates were incubated at ۲۵–۲۷ °C for ۳ days and the growthinhibition was quantified by measuring inhibition zone radius (IZR).Results : The capacity of species including Pantoea stewartii subsp. stewartii, P. agglomerans, P.ananatis, Stenotrophomonas maltophilia, Enterobacter cloacae, Kosakonia cowanii, Pseudomonassp. and sp. Acinetobacter sp. were significantly different. All isolates were able to create aninhibition zone with different radius. Pseudomonas sp. showed the highest sensitivity tobacteriocin production compared to other isolates. The inhibitory zones produced by Pseudomonassp. were larger than other isolates. Two strains of Acinetobacter sp. and P. ananatis had the lowestlevel of bacteriocin production compared to other isolates.Conclusion : The unravelling of the occurrence and roles of bacteriocins from plant pathogens hasonly just begun. The potential advantages of creating more knowledge are obvious, new narrowspectrumantimicrobials may emerge that can contribute to covering agriculture’s need for moresustainable and effective strategies for plant disease control.کلیدواژه ها
Maize, public health, antibiotic-resistant bacteria, bacteriocin.مقالات مرتبط جدید
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