Prophage typing of methicillin-resistant Staphylococcusaureus in the traditional dairy products of Ilam
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_425
- زبان مقاله: انگلیسی
- تعداد مشاهده: 146
نویسندگان
Ilam University
Ilam University
Ilam University
چکیده
Background and Aim : The extensive use of antimicrobial agents in animal husbandry contributesto the selection of drug-resistant strains. The aim of this study was to determine antibioticresistance pattern and investigate some profagetypes, SGFa, SGFb and SGB in methicillinresistantStaphylococcus aureus isolates from traditional dairy specimens in Ilam.Methods : One hundred and sixteen samples were collected from dairy products from ۳۹traditional dairy stores in Ilam. All the samples were tested by using specific biochemical,microscopic to identify S. aureus. Multiplex PCR for the femA and mecA genes was performed toconfirm the identification and methicillin resistance of S. aureus. For prophage typing all S. aureusisolates were screened for SGFa, SGFb and SGB genes by PCR. Susceptibility to ten antibioticswas determined according to CLSI guidelines by using disk diffusion test.Results : Thirty of the samples were identified S. aureus and according to the presence of mecAgene, ۷% were Methicillin Resistance S. aureus (MRSA). The highest resistance to antibiotic wereobserved for doxycycline (۸۷%). For other antibiotics, ۶۷%, ۵۷%, ۴۳%, ۱۶%, ۱۳%, ۱۰% and ۶.۵%resistance were observed to tetracycline, cefexime, cefotaxime, penicillin G, vancomycin,amoxicillin and cefazolin, respectively. The least resistance were seen to imipenem antibiotics(۳%). One S. aureus was positive for SGFa and one isolates positive for SGFb and two isolateswere positive for SGB.Conclusion : Generally, all isolates were resistant to at least for ۳ antibiotics. According to theresults for MRSA isolates and the presence of the prophages gene can be concluded the traditionaldairy product could be a resource of the S. aureus that carry these genes. This may pose a publichealth hazard, since it has been shown that S. aureus that carrying prophage genes and MRSAclone have the zoonotic potential.کلیدواژه ها
MRSA, Prophage, Bacterial resistance, Virulence factorsمقالات مرتبط جدید
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