Genotyping Helicobacter pylori and fgf۷ Gene Expression inGastric cancer
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_316
- زبان مقاله: انگلیسی
- تعداد مشاهده: 93
نویسندگان
Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Institute of Molecular and Cell Biology, Agency of Science, Technology and Research (A*STAR), Singapore
چکیده
Background and Aim : Helicobacter pylori as the causative agent of the most common chronicbacterial infectious disease in human still involves a range of clinical challenging complications.In this meantime, the survey of the interaction between H. pylori virulence genes expression andits consequences on gastric antral epithelial cells is Controversial. This study surveyed thecorrelations between H. pylori cag Pathogenicity Island and virulence factors , genes with Fgf۷gene expression as an angiogenic factor in developing gastric cancer in gastric antral epithelialcells of patients with H. pylori infection.Methods : Gastric antral biopsy samples collected from patients out of exclusion criteria,including consumption of tobacco, alchohol and anti-H. pylori drugs, were categorized into gastriccancer (case group n:۵۳) and gastritis (control group n:۵۰) with and without H. pylori infection todetect changes in cDNA of fgf۷ in gastric antral epithelial cells by using Real Time RT PCR.Extracted total RNA from gastric antral biopsy samples was used to synthesize cDNA for real timePCR. Furthermore, the cDNA of H. pylori cag Pathogenicity Island and other virulence factors ,genes were detected by using specific designed primers and simple PCR.Results : Fgf۷ gene expression revealed a significantly increase in gastric antral epithelial cells ofgastric cancer and H. pylori-positive patients in contrast with gastritis and H. pylori-negativepatients (p< ۰.۰۵). In the meanwhile, cag Pathogenicity Island and hopQ genotypes showed apositive correlation with Fgf۷ gene expression (fold changes of cDNA) in gastric antral epithelialcells (p< ۰.۰۵).Conclusion : This study revealed an obvious correlation between Fgf۷ gene expression in gastricantral epithelial cells of patients with H. pylori carcinogenic genotypes infection and some hostfactors including age.کلیدواژه ها
Fibroblast Growth Factor ۷ gene, Helicobacter pylori, Gastric cancerاطلاعات بیشتر در مورد COI
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