Antimicrobial Efficacy and Prevalence of Microcins amongEscherichia coli isolates

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 88

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

MEDISM23_293

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Bacteriocin is defined as an antibacterial protein with bactericidalactivity. Microcins are a diverse group of low-molecular-weight antibiotics produced by severalEnterobacteriaceae, mainly strains of Escherichia coli. The objective of this study was to detectand evaluate the wide range of antimicrobial activity of Microcins produced by thebacteriocinogenic Escherichia coli.Methods : In this experimental study, ۱۲۵ clinical isolates were selected from private diagnosticlaboratories at Isfahan during the year ۲۰۲۱. They were cultured and incubated at ۳۵°C for ۱۸ - ۲۴hours. Antagonistic activity of isolates was tested by adopting agar plug method. Total DNA wasextracted from clinical specimens and PCR was optimized using specific primers for theamplification of the complete sequence of some Microcin genes.Results : ۱۲۵ isolates of Escherichia coli were isolated from clinical samples. It was shown thatthe antibiotic activity of this peptide is mainly directed to Enterobacteriaceae, including severalpathogenic E. coli strains. Out of ۱۲۵ isolates, ۳۶% (n=۴۵) isolates had positive well assaysamples. Based on the obtained results by the PCR analysis ۴۰%, ۳۳.۳% and ۸.۸% of the isolateswere positive for Mcc J۲۵, Mcc M and Mcc B۱۷ genes respectively. four isolates were Doubleproducers.(Mcc J۲۵ and Mcc M)Conclusion : The objective of the present study was to evaluate the inhibitory activity ofmicrocins, particularly J۲۵ and M, against clinical strains of several Enterobacteriaceae. Ourresults showed that most strains tested were inhibited by at least two microcins. This property canbe useful for antibacterial trials.

نویسندگان

Farzaneh MohammadzadehRostami

Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Sharareh Moghim

Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Saeed Javdan

Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Bahram Nasr Esfahani

Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran