Bioactive Compound, Radical Scavenging and AntibacterialActivity of Aqueous Extract of Flowers of Echium amoenum Fisch. &C.A.Mey. from Guilan – Iran

Background and Aim : Natural products have been a major source of new drugs. Plant used fortraditional medicine contains a wide range of substances that can be used to treat chronic as wellas infectious diseases. This research set to assess phytochemicals in the aqueous extracts of Echiumamoenum Fisch. & C.A.Mey. flowers by qualitative and quantative screening procedures.Methods : The Echium amoenum Fisch. & C.A.Mey. flowers were collected, and the extract wasprovided from aqueous by maceration method. The phytochemical assessment was done applyingstandard methods. Later, in vitro antioxidant activity of aqueous extracts were assayed by ۱,۱-diphenyl-۲-picrylhydrazyl (DPPH۰) free radical scavenging method. Then, the in vitroantibacterial activity of the aqueous extract of flowers of Echium amoenum Fisch. & C.A.Mey.against Staphylococcus aureus (PTCC ۱۱۱۳) and Escherichia coli (PTCC ۱۳۹۹) determined by theminimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assay.Results : The results of the phytochemical screening of aqueous extract of flowers of Echiumamoenum Fisch. & C.A.Mey. were (Flavonoids, Quinones, Saponins and Phenols) presented. Thevalue IC۵۰ of aqueous extract determined ۱۸.۹۵ mg/ml. Also, the aqueous extract of flowers ofEchium amoenum Fisch. & C.A.Mey. showed significant antibacterial activity against both grampositive and gram negative bacteria.Conclusion : Thus, the aqueous extract of flowers of Echium amoenum Fisch. & C.A.Mey. wouldbe helpful for the preparation of pharmaceutically useful drugs to destroy pathogenic microbes.

کلیدواژه ها:

Echium amoenum Fisch. & C.A.Mey. ، Maceration Method ، Saponins ، Staphylococcus aureus ، Infectious Diseases