Evaluation the antifungal efficacy of five Iranian essential oilsagainst fluconazole resistant Candida species isolated from patients withurinary tract candidiasis

Background and Aim : Urinary tract candidiasis has been the most common nosocomial fungalinfection in recent years. Candida species are considered as normal microbial flora, immunedeficiency leads to dysbiosis of microbiome and the opportunistic Candida spp especially Candidaalbicans causes urinary tract infection. In spite of increasing antifungal resistant among fungalspecies the speed of commercialization of new antifungals is low. Therefore it is important tosearch for natural products with high antifungal efficacy and low side effects. The aim of thepresent study was to evaluate antifungal efficacy of five Iranian essential oils against fluconazoleresistant Candida species isolated from patients with urinary tract candidiasis.Methods : Chemical composition of Origanum majorana, Artemisia dracunculus, Cymbopogoncitrate, Cinnamomum verum and Caryophyllus aromaticus essential oils was determined applyinggas chromatography/mass spectroscopy (GC/MS) analysis and antioxidant activity was inspectedwith DDPH assay. Antifungal efficacy was done by broth micro-dilution method according toCLSI protocols.Results : Antifungal properties of esstiential oils against fluconazole resistant Candida speciesshowed that Cinnamomum verum, Caryophillium aromaticus, Artemisia dracunculus, Origanumvulgare and Cymbopogon citratus essential oils had MICs between ۱۲۵ to ۱۷۵ mg/mL (meanvalue: ۱۴۷.۷ _ ۲۵.۵ mg/mL), ۷۰۰ to ۱۰۰۰ mg/mL (mean value: ۷۴۰.۹ _ ۱۰۵.۴ mg/mL), ۱۰۰۰ to۲۰۰۰ mg/mL (mean value: ۱۴۵۴.۵ _ ۵۰۹.۶ mg/mL), ۱۷۳ to ۳۵۰ mg/mL (mean value: ۲۰۸ _ ۵۵.۸mg/mL) and ۱۲۵ to ۱۷۵ mg/mL (mean value: ۱۵۶.۸ _ ۲۴.۶ mg/mL) respectively. All essential oilswere identified fungicide.Conclusion : It is concluded that essential oils are suitable to be used in resistant Candida species,nevertheless at this rate, these products could be applied as adjuvant therapy along withconventional antifungals.

کلیدواژه ها:

nosocomial infections ، urinary tract candidiasis ، essential oils ، antifungal resistant ، Candida spp