The application of the loop-mediated isothermal amplificationmethod for rapid detection of methicillin-resistant Staphylococcusaureus
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_186
- زبان مقاله: انگلیسی
- تعداد مشاهده: 215
نویسندگان
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Microbiology, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran
Department of Microbiology, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran
چکیده
Background and Aim : Methicillin-resistant Staphylococcus aureus (MRSA) is an importantproblem associated with significant mortality and morbidity and is well known as a predominantbacterial pathogen. The aim of this study was to identify MRSA strains. In this study (June ۲۰۱۸to June ۲۰۱۹) isolates of S. aureus were obtained from patients referred to teaching hospitals ofAhvaz, Iran.Methods : All isolates were confirmed by conventional microbiological methods. In the following,antimicrobial susceptibility testing (AST), MRSA screening, PCR detection of MRSA, and LAMPassay were performed.Results : Out of a total of ۱۵۶ staphylococcal isolates, ۱۲۶ isolates were identified as MRSA.Seventy-two (۵۷.۱%) MRSA isolates were recovered from the wound. All MRSA isolates weresensitive to vancomycin, linezolid, teicoplanin, quinupristin-dalfopristin, and tigecycline. Theresults of LAMP showed ۱۰۰% agreement with PCR. The sensitivity and specificity of the LAMPassays for the mecA genes were ۱۰۰% and ۱۰۰%, respectively.Conclusion : The LAMP assay is a rapid and simple method for the identification of MRSA.Because of its performance without the need for specific instrumentation, this method can be easilyemployed in medical centers for the detection of mecA.کلیدواژه ها
LAMP, mecA gene, MRSA, PCR, Rapid detectionمقالات مرتبط جدید
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