Molecular detection of some vancomycin resistance genes ofStaphylococcus aureus strains isolated from clinical samples in Thi-Qarprovince / South of Iraq

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 63

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شناسه ملی سند علمی:

MEDISM23_182

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Glycopeptides such as vancomycin are frequently choice antibiotics forthe treatment of infections caused by methicillin resistant Staphylococcus aureus (MRSA). For thelast years incidence of vancomycin intermediate S. aureus (VISA) and vancomycin resistant S.aureus (VRSA) has been increasing in various parts of the world. The present study was carriedout to find the presence of VISA and VRSA in Thi-Qar province-south of Iraq.Methods : A total ۳۱۵ staphylococcal isolates consisting of ۱۳۰ S. aureus and ۱۸۵ coagulasenegative staphylococci (CoNS) were isolated from different clinical specimens from varioushospitals including AL Hussein teaching hospital, AL Haboubi teaching hospital, AL Musawihospital for children, and Burn center. All S. aureus isolates were subjected to MIC testing (againstvancomycin) on Brain Heart Infusion (BHI) vancomycin screen agar test. Disc diffusion testingfor Ampcillin (۱۰ ug), Oxacillin (۱۰ ug), Gentamicin (۱۰ ug), Amikacin (۳۰ ug) Vancomycin (۳۰ug), and cefoxitin (۳۰ ug) was performed. The presence of mecA, vanA, vanB, vanC, vanD, and۱۶s rRNA genes was evaluated by PCR.Results : Out of ۱۳۰ S. aureus, ۷ strains were found to be vancomycin resistant. Seven strains hadgrowth on BHI vancomycin screen agar (vancomycin ۶ ug/ml). All isolates were positive for mecAgene. vanA, vanB, and vanC were detected in ۳, ۲, and ۲ isolates, respectively. vanD gene was notdetected.Conclusion : Conclusion: The present study for the first time reveals the emergence of VRSAfrom this part of south Iraq and indicates the magnitude of antibiotic resistance in and around thestudy area. The major cause of this may be unawareness and indiscriminate use of broad-spectrumantibiotics.

کلیدواژه ها:

vancomycin ، vancomycin resistant Staphylococcus aureus ، vanA ، vanB ، vanC

نویسندگان

Ali Hussein Shery

Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Seyedeh Elham Rezatofighi

Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Hayder Hussein Jalood

Department of Pharmacy, Mazaya University College, Baghdad, Iraq