Antibiotic resistance assessment and multi-drug efflux pumpsof Enterococcus faecium isolated from clinical specimens
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_180
- زبان مقاله: انگلیسی
- تعداد مشاهده: 76
نویسندگان
Department of Microbiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Department of Microbiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Department of Microbiology, Milad Hospital, Tehran, IR Iran
Department of Microbiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
چکیده
Background and Aim : Enterococcus faecium, common commensal bacteria, is a major cause ofnosocomial infections and public health concerns. Reduced sensitivity to fluoroquinolones ispartly due to efflux pumps. An ongoing challenge is to search for novel inhibitors to block theseefflux pumps and restore antibiotic sensitivity. In this research, we seek to investigate theinteraction of ciprofloxacin and an efflux pump inhibitor (EPI), thioridazine, on the inhibition ofthese efflux pumps.Methods : In this study, a total of ۸۸ isolates of E. faecium from clinical specimens were studiedfrom August ۲۰۱۷ to September ۲۰۱۸. All the isolates were characterized by conventionalphenotypic and molecular methods. The antibiotic resistance profiles and the frequency of effluxpump genes were determined by standard susceptibility and molecular test methods. Minimuminhibitory concentrations (MICs) to ciprofloxacin (CIP) with and without thioridazine weremeasured by micro-broth dilution.Results : The highest antibiotic resistance rate was related to ciprofloxacin, levofloxacin, andimipenem ۹۶.۸%, ۹۴.۳%, and ۹۰.۹% respectively. The highest frequency of efflux pumpdeterminants, efmA was ۶۰(۶۸%), followed by emeA, and efrA and/or efrB genes ۴۸(۵۴.۵%) and۴۵(۵۱%) respectively. This study showed that ۴۱(۴۸.۲%) of the isolates became less resistant to atleast ۲-fold or greater decrease in MIC change in the presence of an efflux pump inhibitor.Conclusion : Our results showed that thioridazine as an efflux inhibitor can enhance the effect ofCIP as an efficient antibiotic and suggested that efrAB, efmA, and emeA efflux pumps can play arole in fluoroquinolone resistance.کلیدواژه ها
Enterococcus faecium, Efflux pumps, Antibiotic resistance, Thioridazineاطلاعات بیشتر در مورد COI
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