effect of lactin-nisin-positive Lactococcus probiotic supernatanton Shigella flexneri biofilm.

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 63

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شناسه ملی سند علمی:

MEDISM23_177

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim : Shigella causes bacillary dysentery, a severe form of dysentery that ismore common in developing countries. The prevalence of shigellosis is also seen in industrializedcountries, especially where it is difficult to maintain proper hygiene . Histopathological analysisof colon biopsies from patients with shigellosis shows destruction of the epithelium, mucosalerosion and typical signs of inflammation. edema and infiltration of polymorphonuclear cells intothe lamina propria.Methods : in this study, samples collected from Tehran Children's Medical Center Hospital wereexamined and studied. To carry out this research, ۱۱۰ human samples from children's diarrhealstool samples were collected from Tehran Children's Medical Center Hospital and transferred toKerry Blair transfer medium and then cultured on different mediums including XLD and Hectonenteric agar mediums. After incubation for ۲۴ hours at ۳۷°C, suspected Shigella colonies wereisolated and then cultured in terms of reactions on TSI medium, lysine medium, citrate, MRVP,SIM and urea. Biochemical tests (ornithine decarboxylation reaction, ONPG test, andelproduction, fermentation of mannitol sugars, etc.) were used to differentiate the species.Results : According to optical density cut off value (ODc) which was ۰.۱۳۸, Shigella flexneribacteria isolated from ۵ patients were non-adherent. ۸ patients were weakly adherent, ۱۴ weremoderately adherent, and ۱۰ were strongly adherent. For subsequent tests, bacteria isolated from۲۴ patients with moderate and strong binding were used. MIC test results According to the obtainedresults, the lowest lethality concentration of the supernatant on Shigella bacteria isolated frompatients was reported as ۴۰ μg/ml. According to the results obtained from this test, positive nisinsupernatant has increased the diameter of the halo in Gentamicin, Chloramphenicol and Cefiximeantibiotics and has changed the bacteria from resistance to these antibiotics.Conclusion : Mirenjad et al. investigated the effect of lactobacillus supernatant on multi-drugresistant isolates of Shigella Sonnei and Sh. Flexneri reviewed. The results showed that L. caseistrongly inhibited the development of pathogen samples. Also, the results showed that ۴ out of ۱۸antibiograms showed complete resistance to pathogen samples through the disc diffusion method

کلیدواژه ها:

lactococous lacticShigella flexneri biofilms

نویسندگان

Mohadesseh Dehghan

Department of Microbiology, Faculity of Life Science, Islamic Azad University, North Tehran Branch, Tehran, Iran

farzaneh Hosseini

assistance professor Verified email at iau-tnb.ac.ir