Detection of NDM-۱ producing Klebsiella pneumoniae ST۱۵and ST۱۴۷ in Iran during ۲۰۱۹-۲۰۲۰
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_165
- زبان مقاله: انگلیسی
- تعداد مشاهده: 219
نویسندگان
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
چکیده
Background and Aim : Carbapenems are employed to treat infections caused by Gram-negativebacteria including Klebsiella pneumoniae. This research is aimed to perform phenotypic detectionof β-lactamases and molecular characterization of NDM-۱ positive K. pneumoniae isolates.Another objective is to investigate NDM-۱ producing K. pneumoniae among children in Iran.From ۲۰۱۹ to ۲۰۲۰, altogether ۶۰ K. pneumoniae isolates were acquired from various patients incertain Iranian hospitals.Methods : Antimicrobial susceptibility testing was performed by disk diffusion and brothmicrodilution methods. In addition, mCIM and eCIM were used to confirm the production ofcarbapenemases and metallo-beta-lactamases (MBLs), respectively. Detection of resistance genesnamely, blaNDM-۱, blaIMP, blaVIM, blaKPC, blaOXA-۴۸-like, blaCTX-M, blaSHV, blaTEM,and mcr-۱ was performed by PCR and confirmed by DNA sequencing. Multilocus sequence typing(MLST) was employed to determine the molecular typing of the strains.Results : According to the findings, the highest rate of carbapenem resistance was detected againstdoripenem ۸۳.۳% (۵۰). Moreover, ۳۱.۷% (۱۹) were resistant to colistin. Further to the above,altogether ۸۰% (۴۸) were carbapenemase-producing isolates and among them ۴۶.۷% (۲۸) of theisolates were MBL and ۳۳.۳% (۲۰) isolates were serine ?-lactamase producer. According to thePCR results, ۱۴ isolates produced blaNDM-۱. Remarkably, four blaNDM-۱ positive isolates weredetected in children. In addition, these isolates were clonally related as determined by MLST(ST۱۴۷, ST۱۵). Altogether ten blaNDM-۱ positive isolates were ST۱۴۷ and four blaNDM-۱positive isolates were ST۱۵.Conclusion : Based on the results, the emergence of NDM-producing K. pneumoniae amongchildren is worrying and hence, it is necessary to develop a comprehensive program to controlantibiotic resistance in the country.کلیدواژه ها
K. pneumoniae; blaNDM-۱ gene; carbapenem-resistance; carbapenemase; colistin;metallo-β-lactamase genesمقالات مرتبط جدید
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