Antifungal susceptibility and evaluation of CDR۱ geneexpression in fluconazole-resistant Candida albicans isolated fromvulvovaginitis
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_155
- زبان مقاله: انگلیسی
- تعداد مشاهده: 163
نویسندگان
Department of Medical Mycology, Faculty of Medicine, Tonekabon Branch of Islamic Azad University, Tonekabon, Iran
Department of Medical Mycology, Faculty of Medicine, Tonekabon Branch of Islamic Azad University, Tonekabon, Iran
Department of Mycology, Pasteur Institute of Iran, Tehran ۱۳۱۶۹۴۳۵۵۱, Iran
Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran ۱۴۱۱۵-۳۳۱, Iran
Department of Mycology, Pasteur Institute of Iran, Tehran ۱۳۱۶۹۴۳۵۵۱, Iran
چکیده
Background and Aim : Vulvovaginal candidiasis (VVC) is one of the most common superficialfungal infections, and studies show that about ۷۵% of women experience VVC at least once intheir lifetime. The objective of this study was to analyse the antifungal susceptibility and the roleof CDR۱ gene overexpression as a mechanism of fluconazole resistance in Candida albicansisolated from vulvovaginitis patients.Methods : The study was performed in ۷۸ women suspected of vulvovaginitis. Isolated Candidaalbicans were identified using the ITS-restriction fragment length polymorphism (ITS-RFLP)technique. Antifungal susceptibility testing was performed for fluconazole, amphotericin B,ketoconazole, and itraconazole according to the CLSI M۲۷-A۳ guideline. CDR۱ gene expressionwas conducted in fluconazole-resistant isolates using real-time PCR.Results : Itraconazole was the most effective drug with MIC۵۰=۰.۲۵ ?g/mL and amphotericinBwas the least effective drug with MIC۵۰=۱ ?g/mL against Candida albicans among four testedantifungals with MIC range between ۰.۰۳-۸ ?g/ml and ۰.۰۶-۸ ?g/ml, respectively. Twelve isolateswere resistant to fluconazole. The overexpression of CDR۱ in fluconazole-resistant isolates wasobserved in ۱.۶۸–۳۲.۸-fold compared to the control strain C. albicans ATCC ۱۰۲۳۱.Conclusion : In isolates with high MIC, CDR۱ gene expression was high. It seems that resistantisolates are increasing and drug susceptibility testing is necessary to choose the appropriate drugand prevent resistance.کلیدواژه ها
Candida albicans, Vulvovaginal candidiasis, CDR۱ gene expression, Antifungalsusceptibilityمقالات مرتبط جدید
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