Genotyping and drug susceptibility testing of Mycobacteriumtuberculosis in Iran: a multi-centre study

Background and Aim : Tuberculosis (TB) is a deadly infection and caused ۱.۴ million deaths in۲۰۱۸. Assessing the geographic distribution of major lineages of Mycobacterium tuberculosis cancontribute greatly to TB control. Mycobacterial interspersed repetitive unit variable numbertandem repeat (MIRU-VNTR) typing is commonly used to differentiate various lineages of M.tuberculosis.Methods : A total of ۲۷۴۷ clinical specimens were collected consecutively from October ۲۰۱۸through June ۲۰۱۹. Clinical isolates were identified as M. tuberculosis using standard biochemicaltests. The standard ۱۵-locus MIRU-VNTR typing was used for the genotyping of clinical isolates.Drug susceptibility testing was performed using the conventional proportion method.Results : From the collected specimens, ۱۰۰ were culture positive for M. tuberculosis. UsingMIRU-VNTR, ۹۹ different patterns were detected among the ۱۰۰ isolates. They were distributedin one cluster comprising two strains and ۹۸ unique patterns. Most of our isolates were similar toNew-۱ and Delhi/CAS strains. Of the M. tuberculosis isolates, ۸۳ (۸۳.۰%) were pan-susceptibleand ۱۷ (۱۷.۰%) were resistant to at least one drug.Conclusion : Our study showed that MIRU-VNTR is a useful method for studying the geneticdiversity of M. tuberculosis isolates in different regional settings and will help the healthauthorities to construct a preventive programme for TB.

کلیدواژه ها:

MIRU-VNTR ، Drug susceptibility testing ، Tuberculosis ، genotyping