Evaluation of the simultaneous exposure to diclofenac andgentamicin on some virulence factors of Pseudomonas aeruginosa
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_121
- زبان مقاله: انگلیسی
- تعداد مشاهده: 146
نویسندگان
Department of Biology, University of Guilan, Rasht, Iran
Department of Biology, University of Guilan, Rasht, Iran
چکیده
Background and Aim : Pseudomonas aeruginosa is a Gram-negative bacterium which is anopportunistic human pathogen capable of causing a wide array of life-threatening infections,including ventilator-associated pneumonia, urinary tract infections, burn, wound injuries, bone andjoint infections. Diclofenac is a non-steroidal anti-inflammatory drug (NSAID) which has shownconsiderable antimicrobial activity. Also, combination therapy of antibiotics with nonsteroidalanti-inflammatory drugs (NSAIDs) has shown significant synergism. This study was conducted toevaluate the effect of simultaneous use of diclofenac and gentamicin on the virulence factors ofclinical isolates of P. aeruginosa.Methods : Bacterial identification and isolation was performed using differential biochemicalassays. The antibiotic resistance pattern was determined by the disk diffusion method and theMinimum Inhibitory Concentration (MIC) of diclofenac and gentamicin against P. aeruginosa wasdetermined by broth microdilution assay. Bacterial swarming and twitching motility of isolatedstrains exposed to diclofenac, gentamicin, and diclofenac + gentamicin at sub- MIC concentrationwere also evaluated.Results : According to the results, the bacterial strains were Gram negative, catalase positive andnon-fermentative rods able to grow in cetrimide agar. The MIC of diclofenac and gentamicin weredetermined ۵۱۲۰ and ۱۲۸ μg/ml. The average inhibition zone around gentamicin disksimpregnated with diclofenac were ۲۳ and ۲۴ mm for pathogenic and ATCC strains, respectively,which were significantly greater than the inhibition zones around the antibiotic alone. Also,bacterial swarming and twitching motility was significantly decreased in the presence of bothdrugs compared with either agent alone.Conclusion : According to the study, diclofenac and gentamicin have a promising synergicactivity against P. aeruginosa which could be used for therapeutic approaches. However, furtherstudies are needed in this area.کلیدواژه ها
diclofenac, Pseudomonas, infection, gentamicin.مقالات مرتبط جدید
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