Molecular evaluation of vancomycin-resistant genes inEnterococcus faecalis isolated from clinical specimens

Background and Aim : Enterococci can cause a variety of nosocomial infections from bacteremiaand bloodstream infections to meningitis, urinary tract infections, and various organs. Nosocomialinfections and antibiotic resistance have increased the importance of enterococci. This study wasperformed to evaluate the molecularity of vancomycin Enterococcus faecalis genes isolated fromclinical specimens using the PCR technique.Methods : In this study, ۱۰۰ urine samples from patients with suspected enterococcal infectionswere collected from different hospitals in Markazi province. Enterococcus species were identifiedby standard methods and antibiotic susceptibility testing was performed according to the standardCLSI disk diffusion method. Then the frequency of vanA and vanB genes was examined by PCR.Results : Out of ۱۰۰ isolated samples, ۳۶ samples of Enterococcus faecalis were identified, andusing the multiplex PCR method to evaluate the presence and frequency of vanA and B genes, in۴ isolates of the vanA gene and ۵ isolates of the vanB gene were observed. The highest frequencywas related to the vanB gene.Conclusion : In general, the results of this study showed that Enterococcus faecalis strains aregenetically diverse, indicating the polyclonal prevalence of strains in clinical specimens. Due tothe abundance of antibiotic-resistant strains especially vancomycin among enterococci, controland preventive measures are necessary.

کلیدواژه ها:

Enterococcus faecalis ، vanB ، Vancomycin ، Real-Time PCR