COMBINATORIAL EFFECTS OF ANTIBIOTICS AND ENZYMESAGAINST BACTERIAL BIOFILMS
- سال انتشار: 1401
- محل انتشار: بیست و سومین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM23_019
- زبان مقاله: انگلیسی
- تعداد مشاهده: 155
نویسندگان
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Background and Aim : Antimicrobial Resistance (AMR) and the formation of biofilms are themain challenges of today's medicine because it has become a global problem that affects thetreatment of multiple infections and impacts public health. Infections caused by these multiresistantor biofilm forming bacteria potentially reduce the possibility of effective therapy; thissituation increases morbidity and mortality and treatment costs, and new and innovative strategiesneed to be devised. Biofilm-dispersing enzymes degrade the extracellular polymeric matrixsurrounding bacterial biofilms, and increase their susceptibility to antibiotics and immune cells.Co-treatment using antibiofilm agents with antibiotics appears to hold great promise as one suchstrategy. In this study the effects of anti-biofilm activity of some enzymes alone and in combinationwith antibiotics from various classes on the degradation of dual-species biofilms of Pseudomonasaeruginosa and Staphylococcus aureus were evaluated.Methods : The efficacy of trypsin, β-glucosidase, and DNase I enzymes on the degradation ofdual-species biofilms of Pseudomonas aeruginosa and Staphylococcus aureus in a wound-likemedium are investigated. Moreover, the reduction of minimum biofilm eradication concentration(MBEC) of meropenem and amikacin was evaluated when combined with enzymes.Results : The minimum effective concentrations of trypsin, β-glucosidase, and DNase I enzymesto degrade biofilms were ۱ μg/ml, ۸ U/ml, and ۱۵۰ U/ml, respectively. Combination of ۰.۱۵ μg/mltrypsin and ۵۰ U/ml DNase I had a significant effect on S. aureus-P. aeruginosa biofilms whichresulted in the dispersal and dissolution of all biofilms. In the presence of the enzymatic mixture,MBECs of antibiotics showed a significant decrease (p < ۰.۰۵), at least ۲.۵-fold.Conclusion : In conclusion, it was illustrated that the combination of trypsin/DNase I enzymestargeting different components of biofilm matrix, can be considered as an anti-biofilm agent andan appropriate candidate to degrade S. aureus-P. aeruginosa biofilms.کلیدواژه ها
Trypsin, β-glucosidase, DNase I, biofilm, Pseudomonas aeruginosa, Staphylococcusaureusمقالات مرتبط جدید
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