Identification of the distinctive genes in the development of very early and late relapsed childhood ALL

Backgrounds: Acute lymphoblastic leukemia (ALL) is the most common type of leukemiadiagnosed in children. Between ۱۵ and ۲۰ percent of ALL patients who are received initialtreatment and achieved complete remission will experience the disease return. even very laterelapses in childhood ALL occurs ۵-۲۰ years after diagnosis. The distinctive gene expressionpattern of these prognostic factors remains poorly understood. Very early relapse of ALL ischaracterized by an increased proliferative capacity of leukemic blasts and up-regulated mitoticgenes.Materials and Methods: We first extracted all the genes involved in an ALL disease from theGEO database (FDR≤۰.۰۵) then we obtained all the miRNAs-mRNAs interactions from themirDIP database. And finally, by using Cytoscape to find the most critical differentiation genes.also by GO, we apply enrichment analysis for these genes.Results: We identified a set of top ۱۰ genes (CBX۵, KAT۶A, PLCB۱, ABI۲, C۱۶orf۷۲, CBFB,TNS۱, SSX۲IP, ZFP۳۶L۲, TIMP۳) differentially expressed in very early relapsed ALL comparedto late relapsed disease and also we find that the most key microRNAs in this regard. Themajority of genes had a function in mitosis.Conclusion: This study suggested that The distinguishing feature of early or late onset of theALL disease can be the key role of these top ۱۰ genes that involved cell cycle pathways and celladherence junctions or in the mitotic phase so can regulation of cell growth and transformation.We also find the most critical microRNA in diseases onset and progression.

کلیدواژه ها:

Acute lymphoblastic leukemia (ALL) ، GEO database ، mirDIP database ، microRNA ، Genes