Bioinformatics study of the important role of S۱۰۰A۸/۹ and inhibition microRNA-۲۲۳ in acute myeloid leukemia inflammation and secondaryinjuries

Backgrounds: Acute myeloid leukemia (AML) is a hematopoietic stem cell malignancycharacterized by ineffective hematopoiesis and excessive proliferation of immature myeloidcells. The role of pro-inflammatory cytokines, chemokines, adhesion molecules, andinflammatory enzymes has been linked with chronic inflammation. S۱۰۰A۸ and S۱۰۰A۹ arecalcium-binding proteins predominantly expressed by neutrophils and monocytes and play keyroles in both normal and pathological inflammation. Both proteins were found to promote tumorprogression through the establishment of premetastatic niches and inhibit antitumor immuneresponses. MiR-۲۲۳ is an evolutionarily conserved anti-inflammatory microRNA primarilyexpressed in myeloid cells. MiR-۲۲۳ post-transcriptionally regulates many genes essential ininflammation, cell proliferation, and invasion. Chronic inflammation has been found to mediate awide variety of diseases, including cardiovascular diseases, cancer, diabetes, arthritis,Alzheimer's disease, pulmonary diseases, and autoimmune diseases.Materials and Methods: In this study, Raw data associated to miRNA-۲۲۳, S۱۰۰A۸/۹ wereextracted from databases TCGA, UCSC Xena, String and GEPIA. Then it was analyzed andevaluated with bioinformatics techniques and software such as cytoscape, MCODE and SPSS۲۶.Results: Inhibition of microRNA-۲۲۳ and expression of S۱۰۰A۸/۹ main causes of inflammationin leukemia. According to this study, In the FEB category, category M۳ shows the lowestexpression of miRNA-۲۲۳, and the expression of S۱۰۰A۸/۹ in the age of ۶۱-۸۰ years has themost expression (respectively ۱۳۴۱.۱۴ and ۱۰۸۷.۸۴ Read per million). The P(-value) of patientsurvival in the graph (miRNA-۲۲۳, S۱۰۰A۸/۹) is equal to ۰.۰۷۵, ۰.۰۸۱ and ۰.۰۸۵, respectivelyConclusion: Bioinformatics analysis shows the importance of the effect of the cancer group andthe patient s age was clearly demonstrated. With targeted control of biomarkers, the severity ofinflammation can be informed in a timely manner, the patient can be treated correctly, andsecondary lung and kidney damage can be prevented. It will greatly help the patient, family andmedical staff, increase the success rate of the struggle for survival in AML inflammation andsecondary injuries.

کلیدواژه ها:

Inflammation ، Acute myeloid leukemia ، miRNA-۲۲۳ ، S۱۰۰A۸/۹ ، Bioinformatics